Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method

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MOGHADAM A.; NAZARIAN SH.

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Journal: Iranian South Medical Journal Year:2017 | Volume:20 | Issue:5 Page(s): 426-436

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Title

Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method

Author(s)

MOGHADAM A. | NAZARIAN SH. | Issue Writer Certificate

Keywords

Salmonella InfantisQ2

ERIC-PCRQ1

Genetic diversityQ2

molecular typingQ2

Abstract

Background: Salmonella is the important bacteria of the Enterobacteriaceae family that are very diverse biochemically and serologically, and mainly transmitted through food. The spread of non-typhoid Salmonella is one of the challenging issues in current research. Therefore rapid diagnosis and typing of the pathogensprovide efficient information about detection and controlling of infection. The aim of this study is typing the clinical strains of Salmonella Infantis. Materials and Methods: In this study, strains of Salmonella Infantis were isolated from different health centers. All of the strains were identified by standard microbiology, biochemical and molecular methods. Genetic relationship between strains was analyzed by ERIC-PCR method. Results: In this study, from 842 stool and blood sample of patients with diarrhea, 48 different strains were isolated which related to Salmonella Infantis. Strains categorized into 14 different groups by genotyping using ERIC-PCR method that highest number of the strains were placed in group 5 (20%, 10 strains). Conclusion: Results of this study demonstrated that strains of Salmonella Infantis which are examined genetically were diverse which can be due to the prevalence of polyclonal strains in human samples. It was also shown that ERIC-PCR method has great differential power for the molecular typing.

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APA: Copy

MOGHADAM, A., & NAZARIAN, SH.. (2017). Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 20(5 ), 426-436. SID. https://sid.ir/paper/33857/en

Vancouver: Copy

MOGHADAM A., NAZARIAN SH.. Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2017;20(5 ):426-436. Available from: https://sid.ir/paper/33857/en

IEEE: Copy

A. MOGHADAM, and SH. NAZARIAN, “Molecular Typing Isolates of Salmonella Enterica Serovar Infantis Using Eric-PCR Method,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 20, no. 5 , pp. 426–436, 2017, [Online]. Available: https://sid.ir/paper/33857/en

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