CONSTRUCTION OF A CLONE EXPRESSING GG OF HUMAN HERPES SIMPLEX VIRUS TYPE 1 IN ORDER TO PRODUCE RECOMBINANT PROTEIN IN PROKARYOTIC SYSTEM

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ZANDI K.; ROUSTAEI M.H.; SADEGHI ZADEH S.; RASAEI M.J.

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Journal: IRANIAN SOUTH MEDICAL JOURNAL (ISMJ) Year:2002 | Volume:5 | Issue:1 Page(s): 1-7

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Title

CONSTRUCTION OF A CLONE EXPRESSING GG OF HUMAN HERPES SIMPLEX VIRUS TYPE 1 IN ORDER TO PRODUCE RECOMBINANT PROTEIN IN PROKARYOTIC SYSTEM

Author(s)

ZANDI K. | ROUSTAEI M.H. | SADEGHI ZADEH S. | RASAEI M.J. | Issue Writer Certificate

Keywords

GLYCOPROTEIN GQ4

HSV-LQ3

CLORIEQ4

GENEQ2

Abstract

Human Herpes Simplex Virus type 1(HSV-1) is the causative agent of herpetic lesions on face, specially around lips and occasionally sexual organs. This virus is also able to cause keratitis and encephalitis in affected hosts. There is high similarity between nucleotide sequences of the genomes of HSV-1 and HSV2, which mostly infect sexual organs. Because of similarity between some surface antigens of these two viruses cross reactions occur in serology tests related to them. Previous experiments have shown that antigenic identity between gGs of these viruses is negligible, and this glycoprotein cap be used as a type specific antigen in serodiagnostic methods of HSV-1. Plasmid pAc-gG-1 containing gG gene of HSV-1 was used in this work to produce recombinant gG. The plasmid was first propagated in its host and DNA was extracted. The plasmid DNAs were digested with restriction enzyme BamHI and gG GENE was isolated and purified. The purified GENE was inserted in the pTrc His2A expression vector correctly. In order to see if the inserted nucleotide sequence of the GENE is in right orientation necessary experiments were done with positive results. This clone was propagated and purified for further works such as protein production.

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APA: Copy

ZANDI, K., ROUSTAEI, M.H., SADEGHI ZADEH, S., & RASAEI, M.J.. (2002). CONSTRUCTION OF A CLONE EXPRESSING GG OF HUMAN HERPES SIMPLEX VIRUS TYPE 1 IN ORDER TO PRODUCE RECOMBINANT PROTEIN IN PROKARYOTIC SYSTEM. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 5(1), 1-7. SID. https://sid.ir/paper/34121/en

Vancouver: Copy

ZANDI K., ROUSTAEI M.H., SADEGHI ZADEH S., RASAEI M.J.. CONSTRUCTION OF A CLONE EXPRESSING GG OF HUMAN HERPES SIMPLEX VIRUS TYPE 1 IN ORDER TO PRODUCE RECOMBINANT PROTEIN IN PROKARYOTIC SYSTEM. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2002;5(1):1-7. Available from: https://sid.ir/paper/34121/en

IEEE: Copy

K. ZANDI, M.H. ROUSTAEI, S. SADEGHI ZADEH, and M.J. RASAEI, “CONSTRUCTION OF A CLONE EXPRESSING GG OF HUMAN HERPES SIMPLEX VIRUS TYPE 1 IN ORDER TO PRODUCE RECOMBINANT PROTEIN IN PROKARYOTIC SYSTEM,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 5, no. 1, pp. 1–7, 2002, [Online]. Available: https://sid.ir/paper/34121/en

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