EXPRESSION OF TGF-β3 IN ISOLATED FIBROBLASTS FROM FORESKIN

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MAHMOUDI RAD MAHNAZ; MAHMOUDI RAD NIKI; MIRDAMADI YASAMAN

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Journal Paper

Paper Information

Journal: REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Year:2015 | Volume:3 | Issue:2 Page(s): 0-0

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Title

EXPRESSION OF TGF-β3 IN ISOLATED FIBROBLASTS FROM FORESKIN

Author(s)

MAHMOUDI RAD MAHNAZ | MAHMOUDI RAD NIKI | MIRDAMADI YASAMAN | Issue Writer Certificate

Keywords

FIBROBLASTS

GENE EXPRESSION

TGF-β3

Abstract

Background: The multifunctional transforming growth factor beta (TGF- b) is a glycoprotein that exists in three isoforms. TGF- b3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. FIBROBLASTS are key cells in the wound healing process. TGF- b3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process. The aim of this study was to express the TGF- b3 gene (tgf-b3) in human foreskin FIBROBLASTS (HFF’s).Methods: We obtained HFF’s from a newborn and a primary fibroblast culture was prepared. The cells were transfected with TGF- b3-pCMV6-XL5 plasmid DNA by both lipofection and electroporation. Expression of TGF-b3 was measured by enzyme-linked immunosor bent assay (ELISA).Results: The highest TGF- b3 expression (8.3-fold greater than control) was obtained by lipofection after 72 hours using 3 ml of transfection reagent. Expression was 1.4-fold greater than control by electroporation.Conclusions: In this study, we successfully increased TGF- b3 expression in primary fibroblast cells. In the future, grafting these transfected FIBROBLASTS onto wounds can help the healing process without scarring.

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APA: Copy

MAHMOUDI RAD, MAHNAZ, MAHMOUDI RAD, NIKI, & MIRDAMADI, YASAMAN. (2015). EXPRESSION OF TGF-β3 IN ISOLATED FIBROBLASTS FROM FORESKIN. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 3(2), 0-0. SID. https://sid.ir/paper/343109/en

Vancouver: Copy

MAHMOUDI RAD MAHNAZ, MAHMOUDI RAD NIKI, MIRDAMADI YASAMAN. EXPRESSION OF TGF-β3 IN ISOLATED FIBROBLASTS FROM FORESKIN. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2015;3(2):0-0. Available from: https://sid.ir/paper/343109/en

IEEE: Copy

MAHNAZ MAHMOUDI RAD, NIKI MAHMOUDI RAD, and YASAMAN MIRDAMADI, “EXPRESSION OF TGF-β3 IN ISOLATED FIBROBLASTS FROM FORESKIN,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 3, no. 2, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/343109/en

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