CONSTRUCTION AND EUKARYOTIC EXPRESSION OF RECOMBINANT LARGE HEPATITIS DELTA ANTIGEN

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FOROUHAR KALKHORAN BEHNAZ; BEHZADIAN FARIDA; SABAHI FARZANEH; KARIMI MOHSEN; MIRSHAHABI HESAM

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Journal: REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Year:2013 | Volume:2 | Issue:1 Page(s): 0-0

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Title

CONSTRUCTION AND EUKARYOTIC EXPRESSION OF RECOMBINANT LARGE HEPATITIS DELTA ANTIGEN

Author(s)

Keywords

HEPATITIS DELTA VIRUS

L-HDAG

SOEING-PCR

Abstract

Background: HEPATITIS DELTA VIRUS (HDV) is a subviral human pathogen that exploits host RNA editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (HDAg). Editing at the amber/W site of HDV antigenomic RNA leads to the production of the large form (L-HDAg), which is required for RNA packaging.Methods: In this study, PCR-based site-directed mutagenesis by the overlap extension method was used to create the point mutation converting the small-HDAg (S-HDAg) stop codon to a tryptophan codon through three stages.Results: Sequencing confirmed the desirable mutation and integrity of the L-HDAG open reading frame. The amplicon was ligated into pcDNA3.1 and transfected to Huh7 and HEK 293 cell lines. Western blot analysis using enhanced chemiluminescence confirmed L-HDAG expression. The recombinant L-HDAG localized within the nuclei of cells as determined by immunofluorescence and confocal microscopy.Conclusion: Because L-HDAG requires extensive post-translational modifications, the recombinant protein expressed in a mammalian system might be fully functional and applicable as a tool in HDV molecular studies, as well as in future vaccine research.

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APA: Copy

FOROUHAR KALKHORAN, BEHNAZ, BEHZADIAN, FARIDA, SABAHI, FARZANEH, KARIMI, MOHSEN, & MIRSHAHABI, HESAM. (2013). CONSTRUCTION AND EUKARYOTIC EXPRESSION OF RECOMBINANT LARGE HEPATITIS DELTA ANTIGEN. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2(1), 0-0. SID. https://sid.ir/paper/343131/en

Vancouver: Copy

FOROUHAR KALKHORAN BEHNAZ, BEHZADIAN FARIDA, SABAHI FARZANEH, KARIMI MOHSEN, MIRSHAHABI HESAM. CONSTRUCTION AND EUKARYOTIC EXPRESSION OF RECOMBINANT LARGE HEPATITIS DELTA ANTIGEN. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2013;2(1):0-0. Available from: https://sid.ir/paper/343131/en

IEEE: Copy

BEHNAZ FOROUHAR KALKHORAN, FARIDA BEHZADIAN, FARZANEH SABAHI, MOHSEN KARIMI, and HESAM MIRSHAHABI, “CONSTRUCTION AND EUKARYOTIC EXPRESSION OF RECOMBINANT LARGE HEPATITIS DELTA ANTIGEN,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 2, no. 1, pp. 0–0, 2013, [Online]. Available: https://sid.ir/paper/343131/en

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