Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

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Safaei Shima; REZVAN HOSSEIN; FATEH ROOHOLLAH; KHALIFEH GHOLI MOHAMMAD

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Journal: JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES Year:2020 | Volume:8 | Issue:2 Page(s): 60-64

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Title

Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Author(s)

Safaei Shima | REZVAN HOSSEIN | FATEH ROOHOLLAH | KHALIFEH GHOLI MOHAMMAD | Issue Writer Certificate

Keywords

Cryptococcus neoformans

Enzyme-Linked Immunosorbent Assay

Heat Shock Protein 70KD

Abstract

Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70 (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit’ s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.

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References

Mechanisms of Pulmonary Escape and Dissemination by Cryptococcus neoformans

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APA: Copy

Safaei, Shima, REZVAN, HOSSEIN, FATEH, ROOHOLLAH, & KHALIFEH GHOLI, MOHAMMAD. (2020). Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, 8(2), 60-64. SID. https://sid.ir/paper/343206/en

Vancouver: Copy

Safaei Shima, REZVAN HOSSEIN, FATEH ROOHOLLAH, KHALIFEH GHOLI MOHAMMAD. Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES[Internet]. 2020;8(2):60-64. Available from: https://sid.ir/paper/343206/en

IEEE: Copy

Shima Safaei, HOSSEIN REZVAN, ROOHOLLAH FATEH, and MOHAMMAD KHALIFEH GHOLI, “Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit,” JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, vol. 8, no. 2, pp. 60–64, 2020, [Online]. Available: https://sid.ir/paper/343206/en

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