PURIFICATION AND EVALUATION OF GLYCEROL TEICHOIC ACID FROM BIOFILM FORMING STAPHYLOCOCCUS EPIDERMIDIS AS A PUTATIVE VACCINE CANDIDATE

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MIRZAEI B.; MOOSAVI S.F.; BABAEI R.; SIADAT S.D.; FARJAH A.; SHAHROOEI M.

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Journal Paper

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Journal: VACCINE RESEARCH Year:2016 | Volume:3 | Issue:1-2 Page(s): 21-25

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Title

PURIFICATION AND EVALUATION OF GLYCEROL TEICHOIC ACID FROM BIOFILM FORMING STAPHYLOCOCCUS EPIDERMIDIS AS A PUTATIVE VACCINE CANDIDATE

Author(s)

Keywords

GLYCEROL TEICHOIC ACID (GLY-TA)

STAPHYLOCOCCUS EPIDERMIDIS

FTIR

NMR

Abstract

Introduction: Biofilm forming STAPHYLOCOCCUS EPIDERMIDIS is a main causative agent of infections related to medical devices. Purification and evaluation of Gly-TA polysaccharide from a biofilm-forming S. epidermidis as a putative vaccine candidate were the main goals of the current study. Methods: Taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (Gly-TA) was purified from the above-mentioned strain and biochemical analyses including, Fourier Transform Infrared spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance spectroscopy (H1-NMR) were conducted for the recovered polysaccharide. Results: Following PCR confirmation of a S. epidermidis strain, Gly-TA was extracted and its biochemical compositions (i. e. N-acetyl glucose amine residues) were obtained. Conclusion: It is envisaged that Gly-TA polysaccharide could be considered as a putative vaccine to inhibit formation of biofilm by S. epidermidis.

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APA: Copy

MIRZAEI, B., MOOSAVI, S.F., BABAEI, R., SIADAT, S.D., FARJAH, A., & SHAHROOEI, M.. (2016). PURIFICATION AND EVALUATION OF GLYCEROL TEICHOIC ACID FROM BIOFILM FORMING STAPHYLOCOCCUS EPIDERMIDIS AS A PUTATIVE VACCINE CANDIDATE. VACCINE RESEARCH, 3(1-2), 21-25. SID. https://sid.ir/paper/344378/en

Vancouver: Copy

MIRZAEI B., MOOSAVI S.F., BABAEI R., SIADAT S.D., FARJAH A., SHAHROOEI M.. PURIFICATION AND EVALUATION OF GLYCEROL TEICHOIC ACID FROM BIOFILM FORMING STAPHYLOCOCCUS EPIDERMIDIS AS A PUTATIVE VACCINE CANDIDATE. VACCINE RESEARCH[Internet]. 2016;3(1-2):21-25. Available from: https://sid.ir/paper/344378/en

IEEE: Copy

B. MIRZAEI, S.F. MOOSAVI, R. BABAEI, S.D. SIADAT, A. FARJAH, and M. SHAHROOEI, “PURIFICATION AND EVALUATION OF GLYCEROL TEICHOIC ACID FROM BIOFILM FORMING STAPHYLOCOCCUS EPIDERMIDIS AS A PUTATIVE VACCINE CANDIDATE,” VACCINE RESEARCH, vol. 3, no. 1-2, pp. 21–25, 2016, [Online]. Available: https://sid.ir/paper/344378/en

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