Cloning and expression of PMI1945 involved in iron acquisition as a promising vaccine candidate against Proteus mirabilis

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KAVEHEI B.; HABIBI M.; SARI S.; ASADI KARAM M.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: VACCINE RESEARCH Year:2018 | Volume:5 | Issue:1 Page(s): 14-18

Download Full-Text

View:

208

Download:

Cites:

Information Journal Paper

Title

Cloning and expression of PMI1945 involved in iron acquisition as a promising vaccine candidate against Proteus mirabilis

Author(s)

KAVEHEI B. | HABIBI M. | SARI S. | ASADI KARAM M.R. | Issue Writer Certificate

Keywords

Proteus mirabilis

Iron scavenger receptors

PMI1945

Cloning

Expression

Abstract

Introduction: Proteus mirabilis is one of the common pathogens of urinary tract infections. Iron scavenger receptors from P. mirabilis are considered as important virulence factors of this strain and have the properties of an ideal vaccine candidate. In this study, the frequency of P. mirabilis iron receptor 1945 (PMI1945) was evaluated in the isolates and then its Expression was conducted in pET28a-BL21. Methods: Amplification of PMI1945 was performed by PCR using P. mirabilis isolates genomic DNA. Cloning of PMI1945 gene was done in pET28a-BL21 system. After transformation, the Expression of the cloned gene was induced by IPTG. The Expression of this protein was then evaluated by SDS-PAGE and Western blot techniques. Results: The frequency of PMI1945 gene in the isolates was 76%. The Cloning of PMI1945 gene into pET28a vector was confirmed by electrophoresis, PCR, enzyme digestion and sequencing. The sequencing of the cloned gene showed 100% identity with other sequences of PMI1945 gene in GenBank. SDS-PAGE and Western blot results showed that 77 kDa PMI1945 protein was successfully expressed in BL21 (DE3) host. Conclusion: Cloning and Expression of PMI1945 was done as the first step for evaluation of a novel vaccine candidate against UTIs caused by P. mirabilis.

Cites

No record.

References

No record.

Cite

APA: Copy

KAVEHEI, B., HABIBI, M., SARI, S., & ASADI KARAM, M.R.. (2018). Cloning and expression of PMI1945 involved in iron acquisition as a promising vaccine candidate against Proteus mirabilis. VACCINE RESEARCH, 5(1), 14-18. SID. https://sid.ir/paper/344423/en

Vancouver: Copy

KAVEHEI B., HABIBI M., SARI S., ASADI KARAM M.R.. Cloning and expression of PMI1945 involved in iron acquisition as a promising vaccine candidate against Proteus mirabilis. VACCINE RESEARCH[Internet]. 2018;5(1):14-18. Available from: https://sid.ir/paper/344423/en

IEEE: Copy

B. KAVEHEI, M. HABIBI, S. SARI, and M.R. ASADI KARAM, “Cloning and expression of PMI1945 involved in iron acquisition as a promising vaccine candidate against Proteus mirabilis,” VACCINE RESEARCH, vol. 5, no. 1, pp. 14–18, 2018, [Online]. Available: https://sid.ir/paper/344423/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops