The Study of MTHFR Gene C677T Polymorphism in Human Embryonic Blastomeres by Fluorescence PCR Method

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SAEEDI SAEEDEH; MASHAYEKHI MOHAMMAD REZA; MALEKI MASOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Crescent Journal of Medical and Biological Sciences Year:2018 | Volume:5 | Issue:3 Page(s): 248-252

Download Full-Text

View:

166

Download:

113

Cites:

Information Journal Paper

Title

The Study of MTHFR Gene C677T Polymorphism in Human Embryonic Blastomeres by Fluorescence PCR Method

Author(s)

SAEEDI SAEEDEH | MASHAYEKHI MOHAMMAD REZA | MALEKI MASOUD | Issue Writer Certificate

Keywords

PGD

PEP-PCR

MTHFR gene

C677T polymorphism

Recurrent miscarriage

Abstract

Objectives: The aim of the present study was to evaluate methylene tetrahydrofolate reductase (MTHFR) gene C677T polymorphism in human pre-implantation embryos. Methods: Fifty embryos after biopsy at cleavage stage (which were inappropriate for assisted reproductive techniques) were analyzed in order to study MTHFR C677T polymorphism and sex determination. After cell lysis, in the first step, whole genome amplification (WGA) was performed by primer extension pre-amplification (PEP) PCR method. In the next step, fluorescence PCR with labeled primers was used to examine this polymorphism in the blastomeres. Finally, the Hinfl enzyme was employed to cut the fragments and the capillary electrophoresis was used to determine the size of PCR products. Results: only 37 blastomeres among 50 samples were successfully amplified; 23 male and 14 female embryos were observed. The frequencies of CC, CT and TT genotypes in embryos were 67. 56%, 24. 32%, 8. 10%, respectively. Additionally, the frequencies of 677C and 677T alleles were 80% and 20%, respectively. Conclusions: In this study, the frequency of MTHFR c. 677C˃ T genotype was evaluated in human embryonic blastomeres before implantation. Therefore, this polymorphism may provide a new and valuable biomarker for evaluating the embryo usefulness and improving the In vitro fertilisation (IVF) process by choosing the best embryo. Our findings revealed that the detection rate of C677T polymorphism was 74% in the samples.

Cites

No record.

References

No record.

Cite

APA: Copy

SAEEDI, SAEEDEH, MASHAYEKHI, MOHAMMAD REZA, & MALEKI, MASOUD. (2018). The Study of MTHFR Gene C677T Polymorphism in Human Embryonic Blastomeres by Fluorescence PCR Method. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, 5(3), 248-252. SID. https://sid.ir/paper/345021/en

Vancouver: Copy

SAEEDI SAEEDEH, MASHAYEKHI MOHAMMAD REZA, MALEKI MASOUD. The Study of MTHFR Gene C677T Polymorphism in Human Embryonic Blastomeres by Fluorescence PCR Method. CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES[Internet]. 2018;5(3):248-252. Available from: https://sid.ir/paper/345021/en

IEEE: Copy

SAEEDEH SAEEDI, MOHAMMAD REZA MASHAYEKHI, and MASOUD MALEKI, “The Study of MTHFR Gene C677T Polymorphism in Human Embryonic Blastomeres by Fluorescence PCR Method,” CRESCENT JOURNAL OF MEDICAL AND BIOLOGICAL SCIENCES, vol. 5, no. 3, pp. 248–252, 2018, [Online]. Available: https://sid.ir/paper/345021/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops