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Title

MULTIPLEX REAL-TIME PCR ASSAY FOR THE DETECTION OF LT, STIA AND STIB GENES IN ENTEROTOXIGENIC ESCHERICHIA COLI

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Abstract

 Background: Diarrhea is an important cause of illness and death among all age groups on a global scale. ENTEROTOXIGENIC ESCHERICHIA COLI (ETEC)protozoa were established as a causative agent of diarrhea in developing and developed countries. The identification of diarrheagenic E. coli (DEC) strains needs to detect the factors that determine the virulence of these organisms. Objectives: In this study, we aimed to use the multiplex real-time (MRT) and multiplex PCR assays for identification of ETEC in patients with diarrhea in SHIRAZ, Iran.Patients and Methods: A total of 430 stool samples were collected from patients with diarrhea in SHIRAZ, in 2012. Diarrheagenic E. coli (DEC) strains were isolated by standard biochemical analysis. We used MRT-PCR and multiplex PCR (MPCR) assays to detect the presence ofLT, STΙa and STΙb genes in ETEC.Results: In this study, 430 stool samples were tested and 52 (12.1%) were identified as contaminated with E. coli using standard biochemical tests. The ETEC were detected in eight patients (15.4%) with diarrhea by the MRT-PCR and MPCR methods. The results of this study showed that four out of eight strains (50%) were STI a producer, and three out of eight strains were ST producers (37.5%). Also, one strain (12.5%) contained both STΙa and LT genes, simultaneously. Conclusions: This is the first study performed in SHIRAZ to identify ETEC intestinal pathogens in patients with diarrhea. The results of this study showed that MRT-PCR can be used as a replacement for the conventional MPCR assay to detect the ETEC strains.

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    APA: Copy

    ABBASI, PEJMAN, KARGAR, MOHAMMAD, MARDANEH, JALAL, DEHYADEGARI, MOHAMMAD ALI, GHORBANI DALINI, SADEGH, & DOOSTI, ABBAS. (2014). MULTIPLEX REAL-TIME PCR ASSAY FOR THE DETECTION OF LT, STIA AND STIB GENES IN ENTEROTOXIGENIC ESCHERICHIA COLI. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 2(1), 0-0. SID. https://sid.ir/paper/347476/en

    Vancouver: Copy

    ABBASI PEJMAN, KARGAR MOHAMMAD, MARDANEH JALAL, DEHYADEGARI MOHAMMAD ALI, GHORBANI DALINI SADEGH, DOOSTI ABBAS. MULTIPLEX REAL-TIME PCR ASSAY FOR THE DETECTION OF LT, STIA AND STIB GENES IN ENTEROTOXIGENIC ESCHERICHIA COLI. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2014;2(1):0-0. Available from: https://sid.ir/paper/347476/en

    IEEE: Copy

    PEJMAN ABBASI, MOHAMMAD KARGAR, JALAL MARDANEH, MOHAMMAD ALI DEHYADEGARI, SADEGH GHORBANI DALINI, and ABBAS DOOSTI, “MULTIPLEX REAL-TIME PCR ASSAY FOR THE DETECTION OF LT, STIA AND STIB GENES IN ENTEROTOXIGENIC ESCHERICHIA COLI,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 2, no. 1, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/347476/en

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