AN EFFICIENT DNA EXTRACTION METHOD FOR LACTOBACILLUS CASEI, A DIFFICULT-TOLYSE BACTERIUM

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Alimolaei Mojtaba; Mehdi Golchin

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Journal Paper

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Journal: International Journal of Enteric Pathogens Year:2016 | Volume:4 | Issue:1 Page(s): 35-40

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Information Journal Paper

Title

AN EFFICIENT DNA EXTRACTION METHOD FOR LACTOBACILLUS CASEI, A DIFFICULT-TOLYSE BACTERIUM

Author(s)

Alimolaei Mojtaba | Mehdi Golchin | Issue Writer Certificate

Keywords

DNA

EXTRACTION

MODIFIED PROTOCOLS

LACTOBACILLUS CASEI

Abstract

Background: There are several protocols to extract DNA from Lactobacillus spp. In the case of L. casei it is harder because of its especial andthick cell wall.Objectives: In this study, nine DNA EXTRACTION protocols (by lysozyme treatment) were evaluated and compared in two categories (traditional and kit-based protocols) and an improved method was presented.Materials and Methods: DNA quantity and quality was determined by spectrophotometry, agarose gel electrophoresis and polymerasechain reaction (PCR).Results: The results revealed that the yield of extracted DNA differed by each protocol (5.8 - 17.1 mg/100 mL), but provided appropriate DNAfor PCR amplification. The modified protocol offered the best total DNA EXTRACTION method when both quality (DNA purity; 1.54 mg) andquantity (DNA yield; 17.1 mg) were considered.Conclusions: We suggest this protocol for effective and inexpensive DNA isolation from L. casei for downstream biological processes suchas PCR.

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APA: Copy

Alimolaei, Mojtaba, & Mehdi, Golchin. (2016). AN EFFICIENT DNA EXTRACTION METHOD FOR LACTOBACILLUS CASEI, A DIFFICULT-TOLYSE BACTERIUM. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 4(1), 35-40. SID. https://sid.ir/paper/347494/en

Vancouver: Copy

Alimolaei Mojtaba, Mehdi Golchin. AN EFFICIENT DNA EXTRACTION METHOD FOR LACTOBACILLUS CASEI, A DIFFICULT-TOLYSE BACTERIUM. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2016;4(1):35-40. Available from: https://sid.ir/paper/347494/en

IEEE: Copy

Mojtaba Alimolaei, and Golchin Mehdi, “AN EFFICIENT DNA EXTRACTION METHOD FOR LACTOBACILLUS CASEI, A DIFFICULT-TOLYSE BACTERIUM,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 4, no. 1, pp. 35–40, 2016, [Online]. Available: https://sid.ir/paper/347494/en

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