COMPARATIVE STUDY OF CAMPYLOBACTER SPP. ISOLATED FROM CHILDREN WITH GASTROENTERITIS IN BAHONAR HOSPITAL, KARAJ, USING PCR AND RFLP

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Abdi Arefeh; HARZANDI NASER; KALANTAR ENAYATOLLAH

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Journal: International Journal of Enteric Pathogens Year:2016 | Volume:4 | Issue:2 Page(s): 0-0

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Title

COMPARATIVE STUDY OF CAMPYLOBACTER SPP. ISOLATED FROM CHILDREN WITH GASTROENTERITIS IN BAHONAR HOSPITAL, KARAJ, USING PCR AND RFLP

Author(s)

Abdi Arefeh | HARZANDI NASER | KALANTAR ENAYATOLLAH | Issue Writer Certificate

Keywords

CHILDREN

GASTROENTERITIS

PCR

RFLP

CAMPYLOBACTER JEJUNI

C. COLI

Abstract

Background: Campylobacter species are responsible for the majority of cases of food-borne GASTROENTERITIS. The sources of the disease outbreaks are often contaminated water or milk, and consumption of undercooked poultry product is the main cause of sporadic campylobacteriosis cases.Objectives: The aims of this study were to determine the prevalence of Campylobacter GASTROENTERITIS in CHILDRENandto differentiate the interfering species using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods at the Bahonar hospital in Karaj, Iran.Patients and Methods: A total of 150 stool samples were collected from CHILDREN under 10 years old during the summer of 2014.PCR was performed using genus- and species-specific primers and RFLP was done usingAluI and TasI enzymes.Results: The results showed the amplification of 400 and 491 bp segments and Campylobacter contamination in 30 (20%) samples; 5 out of 30Campylobacter positive samples (16.66%) were identified as C. jejuni, 20 (66.66%) as C. COLI, 3 (10%) as C. jejuni and C. COLI (mixed infection), and 2 (6.66%) were identified as non-jejuni, non- coli Campylobacter using the PCR method. Following the evaluation of RFLP results, 7 positive samples (23.33%) showed the electrophoretic pattern ofC. jejuni, 21 (70%) showed the electrophoretic pattern ofC. coli, and 2 (6.6%) showed both of the patterns and mixed contamination with jejuni and coli species. The results of digestion withTasI did not show any C. lari or C. upsaliensis patterns.Conclusions: The results of this study showed high percentage of Campylobacter contamination in the tested stool samples. The other surprising finding was the high rate ofCampylobacter coli positive samples; the difference between the results of PCR using species-specific primers (hipo and asp) and the RFLP method (electrophoretic patterns) in some of the positive samples confirms the hypothesis of variations in nucleotide sequences of thehipo primer binding site in some Iranian isolates of Campylobacters.

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APA: Copy

Abdi, Arefeh, HARZANDI, NASER, & KALANTAR, ENAYATOLLAH. (2016). COMPARATIVE STUDY OF CAMPYLOBACTER SPP. ISOLATED FROM CHILDREN WITH GASTROENTERITIS IN BAHONAR HOSPITAL, KARAJ, USING PCR AND RFLP. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 4(2), 0-0. SID. https://sid.ir/paper/347510/en

Vancouver: Copy

Abdi Arefeh, HARZANDI NASER, KALANTAR ENAYATOLLAH. COMPARATIVE STUDY OF CAMPYLOBACTER SPP. ISOLATED FROM CHILDREN WITH GASTROENTERITIS IN BAHONAR HOSPITAL, KARAJ, USING PCR AND RFLP. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2016;4(2):0-0. Available from: https://sid.ir/paper/347510/en

IEEE: Copy

Arefeh Abdi, NASER HARZANDI, and ENAYATOLLAH KALANTAR, “COMPARATIVE STUDY OF CAMPYLOBACTER SPP. ISOLATED FROM CHILDREN WITH GASTROENTERITIS IN BAHONAR HOSPITAL, KARAJ, USING PCR AND RFLP,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 4, no. 2, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/347510/en

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