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Information Journal Paper

Title

SIMULTANEOUS DETECTION OF INVA, STM4497, AND FLIC183 GENES IN SALMONELLA TYPHIMURIUM BY MULTIPLEX PCR METHOD IN POULTRY MEAT SAMPLES IN ZANJAN, IRAN

Pages

  20-23

Abstract

 Background: SALMONELLA TYPHIMURIUM is one of the most important species of Salmonella that is intracellular parasite and attacks host mucus membrane. These bacteria can cause gastroenteritis, and their main transmission route is water, poultry, meat, egg, and raw food. The aim of this study was to detect three virulence genes associated with S. typhimurium named invA, STM4497, and fliC183 genes by MULTIPLEX PCR method.Materials and Methods: 183 samples of poultry were collected from food products in Zanjan (Iran) and cultured in BPW (Buffered Peptone Water) for 18 hr and at 37oC, and in RVS broth (Rappaport Vassiliadis Soya) for 6 hr at 41.5oC. After amplification of genomic DNA by MULTIPLEX PCR method, occurrence of pathogen contamination was checked and compared with standard strain.Results: From the total of 183 collected samples, 52 (28.4%) samples were positive for S. typhimurium. The frequency of STM4497, fliC183, and invA genes were 49 (27%), 3 (2%), and 53 (29%), respectively.Conclusion: Simultaneous detection of invA, STM4497, and fliC183 genes were recognized as a key for detection of S. typhimurium by MULTIPLEX PCR method.

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    APA: Copy

    DOUSANDEH, SHADI, ASAADI TEHRANI, GOLNAZ, AMINI, BAHRAM, & MAZIRI, PARISA. (2016). SIMULTANEOUS DETECTION OF INVA, STM4497, AND FLIC183 GENES IN SALMONELLA TYPHIMURIUM BY MULTIPLEX PCR METHOD IN POULTRY MEAT SAMPLES IN ZANJAN, IRAN. INFECTION EPIDEMIOLOGY AND MICROBIOLOGY (INFECTION EPIDEMIOLOGY AND MEDICINE), 2(4), 20-23. SID. https://sid.ir/paper/348199/en

    Vancouver: Copy

    DOUSANDEH SHADI, ASAADI TEHRANI GOLNAZ, AMINI BAHRAM, MAZIRI PARISA. SIMULTANEOUS DETECTION OF INVA, STM4497, AND FLIC183 GENES IN SALMONELLA TYPHIMURIUM BY MULTIPLEX PCR METHOD IN POULTRY MEAT SAMPLES IN ZANJAN, IRAN. INFECTION EPIDEMIOLOGY AND MICROBIOLOGY (INFECTION EPIDEMIOLOGY AND MEDICINE)[Internet]. 2016;2(4):20-23. Available from: https://sid.ir/paper/348199/en

    IEEE: Copy

    SHADI DOUSANDEH, GOLNAZ ASAADI TEHRANI, BAHRAM AMINI, and PARISA MAZIRI, “SIMULTANEOUS DETECTION OF INVA, STM4497, AND FLIC183 GENES IN SALMONELLA TYPHIMURIUM BY MULTIPLEX PCR METHOD IN POULTRY MEAT SAMPLES IN ZANJAN, IRAN,” INFECTION EPIDEMIOLOGY AND MICROBIOLOGY (INFECTION EPIDEMIOLOGY AND MEDICINE), vol. 2, no. 4, pp. 20–23, 2016, [Online]. Available: https://sid.ir/paper/348199/en

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