مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

PHENOTYPIC AND GENOTYPIC DETECTION OF METALLO-BETA-LACTAMASES AMONG IMIPENEM-RESISTANT GRAM NEGATIVE ISOLATES

Pages

  36-42

Abstract

 Background: IMIPENEM-resistant GRAM NEGATIVE BACTERIA, resulting from metallo-beta-lactamase (MBLs) -producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. Because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams. The prevalence of metallo-beta-lactamase among IMIPENEM-resistant Acinetobacter spp., Pseudomonas spp. and Enerobacteriaceae isolates is determined.Methods: In this descriptive study 864 clinical isolates of Acinetobacter spp., Pseudomonas spp. and Enterobacteriaceae, were initially tested for IMIPENEM susceptibility. The metallo-beta-lactamase production was detected using combined disk diffusion, double disk synergy test, and Hodge test.Then all IMIPENEM resistant isolates were tested by PCR for imp, vim and ndm genes.Results: Among 864 isolates, 62 (7.17 %) were IMIPENEM-resistant. Positive phonetypic test for metallo-beta-lactamase was 40 (64.5%), of which 24 (17.1%) and 16 (9.2%) isolates were Acinetobacter spp. and Pseudomonas spp., respectively. By PCR method 30 (48.4%) of IMIPENEM resistant Acinetobacter, and Pseudomonas isolates were positive for MBL-producing genes. None of the Enterobacteriaceae isolates were positive for metallo-beta-lactamase activity.Conclusion: The results of this study are indicative of the growing number of nosocomial infections associated with multidrug-resistant GRAM NEGATIVE BACTERIA in this region leading to difficulties in antibiotic therapy. Thereby, using of phenotypic methods can be helpful for management of this problem.

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    Cite

    APA: Copy

    MOHAMMADZADEH, MOHAMMAD, TAVAKOLI, MAHNAZ, MOHEBI, ABOLFAZL, & AGHAYI, SAMAD. (2016). PHENOTYPIC AND GENOTYPIC DETECTION OF METALLO-BETA-LACTAMASES AMONG IMIPENEM-RESISTANT GRAM NEGATIVE ISOLATES. JOURNAL OF MEDICAL BACTERIOLOGY, 4(1-2), 36-42. SID. https://sid.ir/paper/349038/en

    Vancouver: Copy

    MOHAMMADZADEH MOHAMMAD, TAVAKOLI MAHNAZ, MOHEBI ABOLFAZL, AGHAYI SAMAD. PHENOTYPIC AND GENOTYPIC DETECTION OF METALLO-BETA-LACTAMASES AMONG IMIPENEM-RESISTANT GRAM NEGATIVE ISOLATES. JOURNAL OF MEDICAL BACTERIOLOGY[Internet]. 2016;4(1-2):36-42. Available from: https://sid.ir/paper/349038/en

    IEEE: Copy

    MOHAMMAD MOHAMMADZADEH, MAHNAZ TAVAKOLI, ABOLFAZL MOHEBI, and SAMAD AGHAYI, “PHENOTYPIC AND GENOTYPIC DETECTION OF METALLO-BETA-LACTAMASES AMONG IMIPENEM-RESISTANT GRAM NEGATIVE ISOLATES,” JOURNAL OF MEDICAL BACTERIOLOGY, vol. 4, no. 1-2, pp. 36–42, 2016, [Online]. Available: https://sid.ir/paper/349038/en

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