IL-23 AND IL-27 GENE EXPRESSION IN THREE BREAST CANCER CELL LINES AND METASTATIC AND NON-METASTATIC LYMPH NODES IN BREAST CANCER PATIENTS

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ESKANDARI A.R.; JABERIPOUR M.; KHODADADI ALI; GHADERI ABAS

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Journal: Koomesh Year:2011 | Volume:12 | Issue:4 (40) Page(s): 428-435

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Information Journal Paper

Title

IL-23 AND IL-27 GENE EXPRESSION IN THREE BREAST CANCER CELL LINES AND METASTATIC AND NON-METASTATIC LYMPH NODES IN BREAST CANCER PATIENTS

Author(s)

ESKANDARI A.R. | JABERIPOUR M. | KHODADADI ALI | GHADERI ABAS | Issue Writer Certificate

Keywords

BREAST NEOPLASMSQ2

GENE EXPRESSIONQ2

INTERLEUKIN-23& 27 HUMANQ2

LYMPH NODESQ2

Abstract

Introduction: There is a great need to identify prognostic and diagnostic cancer bio-markers which can be applied for vaccine and immunotherapy development. The aim of this study was to determine GENE EXPRESSION of IL-23 and IL-27 in two groups: LYMPH NODES of breast cancer patients and three cell lines. Materials and Methods: IL-23 and IL-27 mRNA transcript were investigated in 10 lymph node samples (consisting of 5 metastasis and 5 non metastasis ones) and three cell lines (including of SKBR3, MDA-MB-468 and MCF7) by quantitative real time PCR (Q-PCR) procedure using designed primers, Master Mix reaction containing SYBER green and β actin housekeeping gene.Results: Data showed no significant differences in IL-23 and IL-27 mRNA GENE EXPRESSIONs among metastatic lymph node and non-metastatatic ones. Also, we did not find any significant differences in IL- 23 and IL-27 mRNA expressions in cancer cell lines.Conclusions: Our findings indicate that breast cancer microenvironment has no effect on inflammation via either IL-23 GENE EXPRESSION or Il-27.

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APA: Copy

ESKANDARI, A.R., JABERIPOUR, M., KHODADADI, ALI, & GHADERI, ABAS. (2011). IL-23 AND IL-27 GENE EXPRESSION IN THREE BREAST CANCER CELL LINES AND METASTATIC AND NON-METASTATIC LYMPH NODES IN BREAST CANCER PATIENTS. KOOMESH, 12(4 (40)), 428-435. SID. https://sid.ir/paper/359610/en

Vancouver: Copy

ESKANDARI A.R., JABERIPOUR M., KHODADADI ALI, GHADERI ABAS. IL-23 AND IL-27 GENE EXPRESSION IN THREE BREAST CANCER CELL LINES AND METASTATIC AND NON-METASTATIC LYMPH NODES IN BREAST CANCER PATIENTS. KOOMESH[Internet]. 2011;12(4 (40)):428-435. Available from: https://sid.ir/paper/359610/en

IEEE: Copy

A.R. ESKANDARI, M. JABERIPOUR, ALI KHODADADI, and ABAS GHADERI, “IL-23 AND IL-27 GENE EXPRESSION IN THREE BREAST CANCER CELL LINES AND METASTATIC AND NON-METASTATIC LYMPH NODES IN BREAST CANCER PATIENTS,” KOOMESH, vol. 12, no. 4 (40), pp. 428–435, 2011, [Online]. Available: https://sid.ir/paper/359610/en

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