UTILIZATION OF PARTICULATE AND IMMUNO-MODULATING ADJUVANTS FOR ENHANCEMENT OF IMMUNE RESPONSE AGAINST TUMORS ANTIGENIC MARKERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAFAGHODI M.; JAFARI M.R.; SAJADI S.A.; DIVAN M.; SAMUEL J.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2004 | Volume:6 | Issue:4 (20) Page(s): 14-23

Download Full-Text

Persian Verion

View:

1,089

Download:

Cites:

Information Journal Paper

Title

UTILIZATION OF PARTICULATE AND IMMUNO-MODULATING ADJUVANTS FOR ENHANCEMENT OF IMMUNE RESPONSE AGAINST TUMORS ANTIGENIC MARKERS

Author(s)

Keywords

CANCER VACCINATIONQ4

MUC1Q4

CPG-ODNQ4

PLGA MICROSPHERESQ4

Abstract

Regards to the weak immunigenicity of tumor cells as well as recombinant antigenic markers (like BLP25), adjuvants are needed for enhancement of immune response and its skewing toward cellular immunity. In this research poly (lactide-co-glycolide) (PLGA) microspheres were used as delivery system for a recombinant mucin named mucin 1 (MUC1, BLP25) as a recombinant antigenic cancer marker, and CPG-ODN was used for enhancement of immune response and its biasing toward cellular immunity. PLGA MICROSPHERES encapsulated with BLP25 and CPG-ODN were prepared using a w/o/w emulsion method. Encapsulation of BLP25 was determined by a HPLC method and spectrophotometry at 260 nm was used for quantification of encapsulated CPG-ODN. In vivo immunization studies were performed by SC injections of 20µg BLP25 and 4 µg CPG-ODN in mice (4 animal per group). Group I) Mice immunized with microspheres co-encapsulated with BLP25 and CpG; Group II)Mice immunized with microspheres encapsulated with BLP25 mixed with CpG solution; Group III) Mice immunized with microspheres encapsulated with BLP25. For evaluation of specifity of immune response, T lymphocytes separated from different groups of mice were incubated with different antigens (T Cell Proliferation Assay). IFN-γ and IL-4 cytokines were assayed by a sandwich ELISA method. T lymphocytes separated from group I, showed high proliferation (stimulation index = 25) and high levels of IFN-y interferon (11200±172 pg/ml) which were significantly higher than other two groups (P<0.0001). Co-encapsulation and co-delivery of MUC1 and CPG-ODN produced high cellular (Th1) immunity responses (high levels of lFN-y and no IL-4), indicating the high adjuvanticity potential of CPG-ODN for immunization against cancer markers. Importance of co-encapsulation of antigen and adjuvant in the same delivery system for better adjuvant effect was also approved.

Cites

No record.

References

No record.

Cite

APA: Copy

TAFAGHODI, M., JAFARI, M.R., SAJADI, S.A., DIVAN, M., & SAMUEL, J.. (2004). UTILIZATION OF PARTICULATE AND IMMUNO-MODULATING ADJUVANTS FOR ENHANCEMENT OF IMMUNE RESPONSE AGAINST TUMORS ANTIGENIC MARKERS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 6(4 (20)), 14-23. SID. https://sid.ir/paper/360672/en

Vancouver: Copy

TAFAGHODI M., JAFARI M.R., SAJADI S.A., DIVAN M., SAMUEL J.. UTILIZATION OF PARTICULATE AND IMMUNO-MODULATING ADJUVANTS FOR ENHANCEMENT OF IMMUNE RESPONSE AGAINST TUMORS ANTIGENIC MARKERS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2004;6(4 (20)):14-23. Available from: https://sid.ir/paper/360672/en

IEEE: Copy

M. TAFAGHODI, M.R. JAFARI, S.A. SAJADI, M. DIVAN, and J. SAMUEL, “UTILIZATION OF PARTICULATE AND IMMUNO-MODULATING ADJUVANTS FOR ENHANCEMENT OF IMMUNE RESPONSE AGAINST TUMORS ANTIGENIC MARKERS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 6, no. 4 (20), pp. 14–23, 2004, [Online]. Available: https://sid.ir/paper/360672/en

Related Journal Papers