PREPARATION OF SOMATIC ANTIGEN FROM ASPERGILLUS FUMIGATUS FOR SERODIAGNOSIS OF ASPERGILOSIS

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MIRHENDI S.H.; ZAENI F.; NASR ESFAHANI B.

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2001 | Volume:9 | Issue:3 Page(s): 52-57

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Information Journal Paper

Title

PREPARATION OF SOMATIC ANTIGEN FROM ASPERGILLUS FUMIGATUS FOR SERODIAGNOSIS OF ASPERGILOSIS

Author(s)

MIRHENDI S.H. | ZAENI F. | NASR ESFAHANI B. | Issue Writer Certificate

Keywords

ASPERGILOSISQ3

ASPERGILLUS FUMIGATUSQ3

SOMATIC ANTIGENQ4

Abstract

ASPERGILOSIS is a mycotic infection caused by various species of the genus aspergillus . This disease shows different clinical forms in regard to predisposing Factors and pathegenesis of the disease . Clinical and mycological diagnosis of the disease is unspecific and problematic , but serology can be helpful for diagnosis and for following up the treatment . The aim of this study is preparaytion of SOMATIC ANTIGEN and related antiserum in Iran to perform precipitation tests for diagnosing the noninvasive froms of ASPERGILOSIS . This can cause needlessness from foreign products . Aspergilus fumigatus was cultured in glucose - peptone broth and shaken in 30o c for 3 days. The harvested colonies were homogenized by seasand in mortar and the cell extracts were purified concentrated and injected subcutaneously in rabbits for producing hyperimmune antiserume. Antigens and antisera was evaluated by counterimmunoelectro phoresis test . The prepared antigens had enough antigenesity for producing high titer of antibody in the rabbits . It also reacted with rabbit and human patients, serum . The number and pattern of the precipitation lines were similar and comparable with standard antigen and antiserum

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APA: Copy

MIRHENDI, S.H., ZAENI, F., & NASR ESFAHANI, B.. (2001). PREPARATION OF SOMATIC ANTIGEN FROM ASPERGILLUS FUMIGATUS FOR SERODIAGNOSIS OF ASPERGILOSIS . JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 9(3), 52-57. SID. https://sid.ir/paper/36356/en

Vancouver: Copy

MIRHENDI S.H., ZAENI F., NASR ESFAHANI B.. PREPARATION OF SOMATIC ANTIGEN FROM ASPERGILLUS FUMIGATUS FOR SERODIAGNOSIS OF ASPERGILOSIS . JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2001;9(3):52-57. Available from: https://sid.ir/paper/36356/en

IEEE: Copy

S.H. MIRHENDI, F. ZAENI, and B. NASR ESFAHANI, “PREPARATION OF SOMATIC ANTIGEN FROM ASPERGILLUS FUMIGATUS FOR SERODIAGNOSIS OF ASPERGILOSIS ,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 9, no. 3, pp. 52–57, 2001, [Online]. Available: https://sid.ir/paper/36356/en

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