Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera

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Mahheidari Naimeh; RASHIDIANI JAMAL; ESKANDARI KHADIJEH

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:29 | Issue:179 Page(s): 40-48

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Title

Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera

Author(s)

Mahheidari Naimeh | RASHIDIANI JAMAL | ESKANDARI KHADIJEH | Issue Writer Certificate

Keywords

gold nanoparticlesQ1

apoferritinQ1

antibodyQ1

spectroscopyQ3

Abstract

Background and purpose: Synthesis of homogeneous and equally important nanoparticles is very popular in various sciences. Preparation and use of gold nanoparticles is very important according to their applications in biotechnology. For synthesizing homogeneous gold nanoparticles many substrates and nanostructures have been used. This study aimed at synthesis of gold nanoparticles by apoferritin to be conjugated to antibody for detection of vibrio cholera. Materials and methods: In this study, gold nanoparticles were prepared in apoferritin and compared with apoferritin free gold nanoparticles. Data were characterized by UV-Vis, DLS, and FE-SEM spectroscopy. Results: Findings showed that the synthesized gold nanoparticles in apoferritin are more homogeneous. Conclusion: The distribution of synthesizing gold nanoparticle in presence of apoferritin was so homogenous. In the following step, gold nanoparticles were separated from apoferritin and conjugated to recombinant antibody for detecting vibrio cholera. By bacteria aatchment on gold nanoparticles, red shift ocured on wavelength, which was also visible to naked eyes. So, gold nanoparticles could be considered as a colored biosensor for detection of vibrio cholera.

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APA: Copy

Mahheidari, Naimeh, RASHIDIANI, JAMAL, & ESKANDARI, KHADIJEH. (2019). Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 29(179 ), 40-48. SID. https://sid.ir/paper/363784/en

Vancouver: Copy

Mahheidari Naimeh, RASHIDIANI JAMAL, ESKANDARI KHADIJEH. Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;29(179 ):40-48. Available from: https://sid.ir/paper/363784/en

IEEE: Copy

Naimeh Mahheidari, JAMAL RASHIDIANI, and KHADIJEH ESKANDARI, “Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 179 , pp. 40–48, 2019, [Online]. Available: https://sid.ir/paper/363784/en

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