THE EFFECT OF PLATELET LAYSET ON GROWTH AND SURVIVAL OF OOCYTES AND PRE-ANTRAL FOLLICLES OF PREPUBERTAL NMRI MOUSE

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PAZOKI H.; EIMANI H.; FAROKHI H.; SHAHVERDI A.H.; EBRAHIMI B.; TAHAEI L.

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2014 | Volume:22 | Issue:3 Page(s): 1265-1273

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Title

THE EFFECT OF PLATELET LAYSET ON GROWTH AND SURVIVAL OF OOCYTES AND PRE-ANTRAL FOLLICLES OF PREPUBERTAL NMRI MOUSE

Author(s)

Keywords

FETAL BOVINE SERUM (FBS)Q2

IN VITRO FOLLICULOGENESISQ2

PLATELET LAYSET (PL)Q2

PRE-ANTRAL FOLLICLEQ2

Abstract

Introduction: Elements present in the culture medium play important roles in cell culturing and vitro follicle culture. In recent years, a great deal of research has been focused to improve the culture medium. In this study, the effect of platelet layset extraction was investigated on growth and survival of oocyte and follicle.Methods: PRE-ANTRAL FOLLICLEs of prepubertal NMRI mouse were prepared with diameter of 100-130mm and cultured for 12 days in different culture mediums. Culture mediums in experimental groups were enriched by 5 and 10% PL, whereas those of two control groups were prepared by 5 and 10% FBS. Each culture medium was refreshed every other day. Eventually, survival rate and growth of follicles were evaluated by inverted microscope after one day and at the end of culturing.Results: Oocytes in medium with PL had significant growth and reached to size of mature oocytes in medium with FBS (p˂0.05). Oocytes diameter reached to 80.62±3.59 and 80.31±4.26 mm after 12 days in medium with 5 and 10%PL compared to those of oocytes in 5 and 10% FBS which were measured as 82.18±2.56 and 82.12±2.58 mm respectively. The number of follicles had significant decrease in medium with10% FBS and experimental groups (5& 10%PL) compared to 5% FBS (p˂0.05). After 12 days, best survival rate belonged to 5%PL and the number of follicles declined significantly in control groups and 10%PL (p˂0.05).Discussion: platelet layset improved, oocyte growth and follicle survival and culture medium of follicle. Therefore, PL can be considered as an appropriate replacement for common serums such as FBS in the future.

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APA: Copy

PAZOKI, H., EIMANI, H., FAROKHI, H., SHAHVERDI, A.H., EBRAHIMI, B., & TAHAEI, L.. (2014). THE EFFECT OF PLATELET LAYSET ON GROWTH AND SURVIVAL OF OOCYTES AND PRE-ANTRAL FOLLICLES OF PREPUBERTAL NMRI MOUSE. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 22(3), 1265-1273. SID. https://sid.ir/paper/36402/en

Vancouver: Copy

PAZOKI H., EIMANI H., FAROKHI H., SHAHVERDI A.H., EBRAHIMI B., TAHAEI L.. THE EFFECT OF PLATELET LAYSET ON GROWTH AND SURVIVAL OF OOCYTES AND PRE-ANTRAL FOLLICLES OF PREPUBERTAL NMRI MOUSE. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2014;22(3):1265-1273. Available from: https://sid.ir/paper/36402/en

IEEE: Copy

H. PAZOKI, H. EIMANI, H. FAROKHI, A.H. SHAHVERDI, B. EBRAHIMI, and L. TAHAEI, “THE EFFECT OF PLATELET LAYSET ON GROWTH AND SURVIVAL OF OOCYTES AND PRE-ANTRAL FOLLICLES OF PREPUBERTAL NMRI MOUSE,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 3, pp. 1265–1273, 2014, [Online]. Available: https://sid.ir/paper/36402/en

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