Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma

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HAGHIRALSADAT BIBI FATEMEH; AMOABEDINY GHASEM; NADERINEZHAD SAMIRA; Morteza Ratki Farzane; ZANDIEH DOULABI BEHROUZ

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2019 | Volume:26 | Issue:12 Page(s): 1027-1037

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Title

Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma

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Keywords

SiRNA lipoplexQ1

Physico-chemical characterizationQ1

Intracellular uptakeQ1

Cellular viabilityQ1

Abstract

Introdution: Cationic liposomes have been presented for gene delivery as an alternative vector instead of viral vectors. A major challenge associated with siRNA delivery is the instability of liposomes, which is still a serious problem. The aim of this study was to provide an appropriate formulation to overcome this instability. Methods: In the present study (Scientific-Fundamental, Experimental-Laboratory Study), liposomal formulation containing soy phosphatidylcholine, cationic DOTAP, cholesterol and polyethylene glycole was synthesized by thin-film hydration method and the siRNA were loaded on liposomes through incubation. In the following; the optimization of siRNA loading was on the agenda. Then the parameters related to size, zeta potential, polydispersity index and lon-term stability of siRNA-liposomes complex were reported. The Data were analyzed by GraphPad Prism version 7 Software. All data were repeated three times and reported as mean± standard deviation. Results: In this study we were able to produce SiRNA lipoplex with high loading efficiency of siRNA. The produced nanoparticles did not agglomerate and were stable at 4 oC for 3 months. This nanosystem could successfully deliver siRNA to normal bone cells. Studies have shown that the blank system (no gene) had no toxicity. Conclusion: The prepared PEGylated liposomes have a great potential for delivery of siRNA to bone cells.

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APA: Copy

HAGHIRALSADAT, BIBI FATEMEH, AMOABEDINY, GHASEM, NADERINEZHAD, SAMIRA, Morteza Ratki, Farzane, & ZANDIEH DOULABI, BEHROUZ. (2019). Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 26(12 ), 1027-1037. SID. https://sid.ir/paper/36416/en

Vancouver: Copy

HAGHIRALSADAT BIBI FATEMEH, AMOABEDINY GHASEM, NADERINEZHAD SAMIRA, Morteza Ratki Farzane, ZANDIEH DOULABI BEHROUZ. Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;26(12 ):1027-1037. Available from: https://sid.ir/paper/36416/en

IEEE: Copy

BIBI FATEMEH HAGHIRALSADAT, GHASEM AMOABEDINY, SAMIRA NADERINEZHAD, Farzane Morteza Ratki, and BEHROUZ ZANDIEH DOULABI, “Formulation of a therapeutic cationic liposome-siRNA complex for development to fight osteosarcoma,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 12 , pp. 1027–1037, 2019, [Online]. Available: https://sid.ir/paper/36416/en

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