Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles

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AFSHARI M.; Sadat Shandiz s.a.; HAMDI S.M.M.

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Journal: JOURNAL OF CELL & TISSUE Year:2020 | Volume:11 | Issue:1 Page(s): 44-54

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Title

Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles

Author(s)

AFSHARI M. | Sadat Shandiz s.a. | HAMDI S.M.M. | Issue Writer Certificate

Keywords

Zinc Oxide nanoparticlesQ1

Breast cancerQ1

Glutation peroxidaseQ1

Glutation reductaseQ1

Abstract

Aim: The aim of the current work was to investigate the anticancer activities of Zinc Oxide nanoparticles (ZnONPs) through modulation of Glutation peroxidase and Glutationreductase gene expression in Breast cancer cells. Material and Methods: In this investigation, the Breast cancer MCF-7 and normal HEK293 cell lines were treated with various concentrations of ZnONPs for overnight. Cell viability was measured using MTT(3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay against cancer and normalcells. The quantity of Glutation peroxidase and Glutationreductase genes compared to GAPDH gene expressions were evaluated using the real time PCR method. Results: The MTT data showed that ZnONPs significantly decreased the viability of cancer cells compared to normal cells in dose-dependent mode. Moreover, the mRNA levels of Glutation peroxidase and Glutation reductase genes was significantly increased by 2. 13± 0. 07 (P<0. 001) and 1. 22± 0. 05 (P<0. 05) fold, respectively, following treatment withZnONPs. Conclusion: According to the results of this investigation, the Glutation peroxidase and Glutation reductase gene expression was the key factors of glutathione system in elimination of increasing reactive oxygen species treated with ZnONPs.

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APA: Copy

AFSHARI, M., Sadat Shandiz, s.a., & HAMDI, S.M.M.. (2020). Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles. JOURNAL OF CELL & TISSUE, 11(1 ), 44-54. SID. https://sid.ir/paper/364451/en

Vancouver: Copy

AFSHARI M., Sadat Shandiz s.a., HAMDI S.M.M.. Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles. JOURNAL OF CELL & TISSUE[Internet]. 2020;11(1 ):44-54. Available from: https://sid.ir/paper/364451/en

IEEE: Copy

M. AFSHARI, s.a. Sadat Shandiz, and S.M.M. HAMDI, “Evaluation of Glutation Peroxidase and Glutation Reductase gene expression against breast cancer cell line (MCF-7) treated with the Zinc Oxide Nanoparticles,” JOURNAL OF CELL & TISSUE, vol. 11, no. 1 , pp. 44–54, 2020, [Online]. Available: https://sid.ir/paper/364451/en

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