JOURNAL OF CELL & TISSUE
Year:2020 | Volume:11 | Issue:1|Papers Count:6

Aim: The aim of this study was to determine the effect of Carob seedextract as natural antioxidant on quality cryopreserved Farahani rams breeding sperm. Material and Methods: In this study semen was collected from five mature ram (weight: 60± 5 kg) twice a week using an artificial vagina and the ejaculates were pooled in order to eliminate the individual effect of rams. Different levels of carob seed extracts (0, 0/05, 0/1, 0/15 and 0/2 mL) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen and with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time. Thereafter and after thawing and incubation for5 min, sperm quality parameters includingmotility, progressive motility, viability (Nigrosine– eosin staining), membrane integrity with Hypoosmotic (Host) and morphology abnormality (Hancock test). Results: Results indicated that the level of 0/05 mL carob seedextract significantly improved some parameters including motility, viability, plasma membrane and morphology integrity compared to control (P<0. 01). Conclusion: Results indicated that so added 0/05 of carob seed extract peel to Tris based extender was beneficial in storage in sperm Farahani ram breeding after freeze-thawing.

Aim: The purpose of this research was aimed to evaluate the protective effects of crocin, as an antioxidant agent on Mast cells, blood vessels and biochemical changes of ovary and serum in Busulfan-induced oxidative stress in Mice. Material and Methods: Thirty mature 6-8 weeks aged female NMRI mice in the weight of 22-25 g were randomly divided into 6 groups, and treated for 21 days. The control group only received solvent of Busulfan (BSF) (0. 1 ml) intraperitoneally, and BSF group received only Busulfan (10 mg -1, IP/single dose). The experimental groups no. 1, 2, 3 received BSF (10 mg kg -1 /day, IP) and positive group only received crocin (400 mg kg /single dose) with crocin (100, 200, 400 mg -1 kg, IP/day). At the end of treatment period, animals were euthanized and left ovary were studied for Mast cells, ovary blood vessels, and Serum, and right ovary for biochemical evaluations. Data was subjected to one‒ way analysis of variance (ANOVA) and Tukey to determine if significant difference (P≤ 0. 01) existed among the observed results using SPSS. Results: Busulfan significantly (P≤ 0. 01) increased mast cells and MDA, while decreased ovary blood vessels and SOD rate, significantly (P=0. 000) in comparison to control group. However, crocin in all the used doses, especially in the dose of 200 mg kg-1, significantly decreased the adverse effects of Busulfan. Conclusion: The results indicated that crocin can protect ovaries against Busulfan induced damages, and it can be considered as a suitable drug for reducing the toxic effects of Busulfan in chemotherapy.

Aim: The aim of this study is to evaluate the interaction of bacterial inoculation and iron treatment (nano and Fechelate) on physiological traits of alfalfa. Material and Methods: In this study, effects of inoculation with standard Rhizobium meliloti, effects of different levels of iron (Fe-chelate, 0, 5, 10, 20 and 25 μ M Fe2O3 nanoparticles) and the interaction of bacterial inoculation and iron treatment were investigated on alfalfa in a factorial experiment in completely randomized design with three replications for 45 days. The measured traits were growth indexes, photosynthetic pigments, protein, proline, antioxidants activity, DPPH(diphenyl-picryl-hydrazyl)-radical scavenging activity percent and elements content. Results: Rhizobium incoculation alone showed beneficial effects on the alfalfa growth and was caused increasing in growth parameters, pigmants, protein content, potassium and phosphours uptake. However inoculation did not effect on the proline and antioxidant content. Iron treatment had a positive effect on the growth parameters, pigmants, protein content and elemant uptake. Highest values of growth parameters was observed 25μ M Fe2O3 nanoparticles. The highest values of proline and antioxidants activity were measured in control (0μ M nanoparticles). Since this concentration is considered a stress for alfalfa. Negative effects of 0μ M nanoparticles decreased in inoculated alfalfa plants with R. meliloti. Indeed rhizobium causes increasing in inoculated plant resistant by reducing stressful conditions. Conclusion: Rhizobium-alfalfa symbiosis plus iron nanofertilizer can cause increasing in plant resistance to stress, in addition to increase growth of plant. The highest amount of growth parameters, pigmants and protein content was measured in inoculated plant with Rhizobium meliloti and 10μ M nanoparticles.

Aim: The aim of the current work was to investigate the anticancer activities of Zinc Oxide nanoparticles (ZnONPs) through modulation of Glutation peroxidase and Glutationreductase gene expression in breast cancer cells. Material and Methods: In this investigation, the breast cancer MCF-7 and normal HEK293 cell lines were treated with various concentrations of ZnONPs for overnight. Cell viability was measured using MTT(3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) assay against cancer and normalcells. The quantity of Glutation peroxidase and Glutationreductase genes compared to GAPDH gene expressions were evaluated using the real time PCR method. Results: The MTT data showed that ZnONPs significantly decreased the viability of cancer cells compared to normal cells in dose-dependent mode. Moreover, the mRNA levels of Glutation peroxidase and Glutation reductase genes was significantly increased by 2. 13± 0. 07 (P<0. 001) and 1. 22± 0. 05 (P<0. 05) fold, respectively, following treatment withZnONPs. Conclusion: According to the results of this investigation, the Glutation peroxidase and Glutation reductase gene expression was the key factors of glutathione system in elimination of increasing reactive oxygen species treated with ZnONPs.

Aim: The aim of this study was to investigate the effects of inoculation of two species of mycorrhizal fungi on growth, colonization percentage, antioxidant enzymes activity, health and risk index of coriander under cadmium stress. Material and methods: A pot experiment was conducted in a completely randomized design with 2 factors and 3 replications in the research greenhouse of the Department of Horticultural Science and Landscape Engineering at the Ferdowsi University of Mashhad in 2018. The first factor was cadmium nitrate at 4 levels of 0, 20, 40, and 80 mg kg soil and the second factor was mycorrhiza application at 3 levels of non-inoculation, inoculation with Glomus mosseae and Glomus intraradices. Results: The results showed that with increasing cadmium concentration in the soil, the shoot and total biomass of plant, leaf area, number of seeds, 1000 seeds weight, colonization percentage, soluble protein, catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase activities were significantly decreased in coriander. But malondialdehyde content, health and risk index increased. However, the use of mycorrhizal fungi reduced the harmful effects of cadmium in the plant. This resulted in a decrease of 47. 1% of the risk and health index and 17. 2% of malondialdehyde in the plant. Conclusion: According to the findings, use of mycorrhizal fungi had a significant effect on the improving of harmful effects of cadmium in coriander, thus improved the risk and health index for consumers. Therefore, using mycorrhizal fungi as a management strategy in polluted areas with this heavy metal is recommended.

Aim: The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines (Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-venerisand apricot kernel) against breast cancer cells (Mcf-7). Material and method: For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator. Different concentrations of herbal extracts (12. 5, 25, 50 and 100 μ g/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange-ethidium bromide staining, respectively. Data was analyzed by SPSS software at the significant level of 5%. Results: Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (P<0. 05) compared to other concentrations of the same extracts. Also, among the high concentrations (100μ g/ml), the highest cell cytotoxicity effects were related to the extracts of Echinacea purpureaand Adiantum capillus-veneris (P<0. 05). Conclusion: The results of this study indicated that the addition of Echinacea purpurea and Adiantum capillus-venerisextractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations.