Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line

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Kheirandish Mohammad Hassan; ZAREI JALIANI HOSSEIN; IMANI MEHDI

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Journal Paper

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2019 | Volume:27 | Issue:7 Page(s): 1735-1746

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Information Journal Paper

Title

Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line

Author(s)

Kheirandish Mohammad Hassan | ZAREI JALIANI HOSSEIN | IMANI MEHDI | Issue Writer Certificate

Keywords

Pore-forming toxinQ1

Recombinant proteinQ2

Fragaceatoxin CQ1

Breast cancerQ2

Abstract

Introduction: Chemotherapy has been restricted due to the high-dose side effects. In the present study, acceleration of the chemotherapeutic drug (5FU) entrance into MCF-7 cells has been explored by using a recombinant form of Fragaceatoxin C (FraC) Pore-forming toxin. Methods: In this experimental study, the gene for FraC toxin was order from a commercial source and was sub-cloned into pET28a vector using NcoI and XhoI restriction enzymes. Expression of the protein was induced by IPTG and purification was performed using Ni-NTA affinity chromatography. Hemolysis was tested using a turbidimetric assay. Cytotoxic and potentiation effects of the recombinant toxin on 5FU chemotherapeutic drug were analyzed using trypan blue and MTT assays. T-student test was used to analyze the data using the SPSS v16 software. Significance was defined as P≤ 0. 05 Results: Recombinant FraC toxin was produced in E. coli with 86% purity. Cytotoxic effects of the FraC toxin in MCF-7 cells were dose and time-dependent. the IC50 of FraC toxin on MCF-7 cells was 3. 3 mg/ml. Non-toxic concentrations of recombinant FraC toxin enhanced cytotoxic effects of 5FU by a factor of 4 to 6 in different exposure times (24-72 h). Conclusion: Potentiation of the 5FU chemotherapeutic effect by acceleration of its entrance into cancer target cells using FraC Pore-forming toxin can reduce its side effects of the chemotherapy.

Cites

Cytotoxic Effect of Recombinant Fragaceatoxin C on Peripheral Blood Mononuclear Cells

References

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APA: Copy

Kheirandish, Mohammad Hassan, ZAREI JALIANI, HOSSEIN, & IMANI, MEHDI. (2019). Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 27(7 ), 1735-1746. SID. https://sid.ir/paper/36498/en

Vancouver: Copy

Kheirandish Mohammad Hassan, ZAREI JALIANI HOSSEIN, IMANI MEHDI. Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;27(7 ):1735-1746. Available from: https://sid.ir/paper/36498/en

IEEE: Copy

Mohammad Hassan Kheirandish, HOSSEIN ZAREI JALIANI, and MEHDI IMANI, “Potentiation Effect Of 5FU by Fragaceatoxin C Pore-Forming Toxin in MCF-7 Cell Line,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 7 , pp. 1735–1746, 2019, [Online]. Available: https://sid.ir/paper/36498/en

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