DEVELOPMENT AND APPLICATION OF A SENSITIVE MULTIPLEX REAL-TIME RT-PCR FOR SIMULTANEOUSLY DETECTION OF HIV-1 AND HCV IN PLASMA SAMPLES

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PARYAN M.; MONDANIZADEH M.; MOHAMMADI YEGANEH S.; KHANSARINEJAD B.

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Journal: Journal of Arak University Medical Sciences Year:2011 | Volume:14 | Issue:5 (58) Page(s): 1-10

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Title

DEVELOPMENT AND APPLICATION OF A SENSITIVE MULTIPLEX REAL-TIME RT-PCR FOR SIMULTANEOUSLY DETECTION OF HIV-1 AND HCV IN PLASMA SAMPLES

Author(s)

PARYAN M. | MONDANIZADEH M. | MOHAMMADI YEGANEH S. | KHANSARINEJAD B. | Issue Writer Certificate

Keywords

HCVQ2

HIV-1Q2

MULTIPLEXQ2

REAL-TIME PCRQ2

SYBR GREENQ2

Abstract

Background: HIV-1 and HCV are two of the most important blood-borne infectious agents. Hence, reliable, precise, and sensitive detection of these viruses in infected patients and donated blood units is highly important. Noticing the limitations of serological assays in detection of these infectious agents, this study was to use fast and sensitive molecular assays like REAL-TIME PCR.Materials and Methods: In this trial, a home-brewed SYBR GREEN-based MULTIPLEX real time PCR, on the basis of melting curve analysis, was developed for the single or simultaneous detection of HCV and HIV-1 infections in plasma samples. Data were analyzed using SPSS software version 16.Results: The results obtained from different reactions on several clinical samples showed that the analytical sensitivities of the developed assay for HIV-1 and HCV were 200 and 100 copies/ml, respectively. It was also shown that the primers designed for each virus had no interaction with each other and other interfering agents.Conclusion: Noticing the good level of sensitivity and specificity, easy handling, relatively low cost, and rapid analysis of samples, this method can be a useful and rapid approach for simple and effective detection of HCV and HIV-1 in plasma samples.

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APA: Copy

PARYAN, M., MONDANIZADEH, M., MOHAMMADI YEGANEH, S., & KHANSARINEJAD, B.. (2011). DEVELOPMENT AND APPLICATION OF A SENSITIVE MULTIPLEX REAL-TIME RT-PCR FOR SIMULTANEOUSLY DETECTION OF HIV-1 AND HCV IN PLASMA SAMPLES. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 14(5 (58)), 1-10. SID. https://sid.ir/paper/365158/en

Vancouver: Copy

PARYAN M., MONDANIZADEH M., MOHAMMADI YEGANEH S., KHANSARINEJAD B.. DEVELOPMENT AND APPLICATION OF A SENSITIVE MULTIPLEX REAL-TIME RT-PCR FOR SIMULTANEOUSLY DETECTION OF HIV-1 AND HCV IN PLASMA SAMPLES. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2011;14(5 (58)):1-10. Available from: https://sid.ir/paper/365158/en

IEEE: Copy

M. PARYAN, M. MONDANIZADEH, S. MOHAMMADI YEGANEH, and B. KHANSARINEJAD, “DEVELOPMENT AND APPLICATION OF A SENSITIVE MULTIPLEX REAL-TIME RT-PCR FOR SIMULTANEOUSLY DETECTION OF HIV-1 AND HCV IN PLASMA SAMPLES,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 14, no. 5 (58), pp. 1–10, 2011, [Online]. Available: https://sid.ir/paper/365158/en

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