Statistical optimization of Cellulase production by Bacillus aerius AV10 in solid-state fermentation of cellulosic wastes and it, s partial characterization

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Azadian Kharanjani f.; BADOEI DALFARD A.; KARAMI Z.

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Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:31 | Issue:4 Page(s): 0-0

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Title

Statistical optimization of Cellulase production by Bacillus aerius AV10 in solid-state fermentation of cellulosic wastes and it, s partial characterization

Author(s)

Azadian Kharanjani f. | BADOEI DALFARD A. | KARAMI Z. | Issue Writer Certificate

Keywords

Plackett-Burman designQ1

ThermophilicQ1

Solid-state fermentationQ1

Wheat strawQ3

Abstract

Cellulases are important industrial enzymes for the most bioconversion processes. In this study, Bacillus aerius isolated and identification as the best Thermophilic cellulose degrading bacterium from Gorooh hot spring and was identified as Bacillus aerius AV10 based on 16S rRNA sequence homology and microbial-biochemical tests. Cultivation conditions of cellulase production by Bacillus aerius AV10 in Solid-state fermentation (SSF) were investigated. The moisture content, yeast extract, MgSO4 and initial culture pH were identified by Plackett-Burman design (PBD) as the significant factors for endoglucanase, exoglucanase and filter paper (FPA) activities. Response surface Methodology and central composite design by 5-level and 4-factor were used for the optimization of enzyme. The optimal ranges of moisture content, yeast extract, MgSO4 and initial culture pH were 61. 4%, 1. 65%, 0. 29% and 6. 2, respectively. Under the optimized conditions, maximum CMCase production was 561. 12 U/ml. The optimal pH and temperature of the enzyme for Wheat straw hydrolysis were determined to be 7. 0 and 60 ° C.

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APA: Copy

Azadian Kharanjani, f., BADOEI DALFARD, A., & KARAMI, Z.. (2019). Statistical optimization of Cellulase production by Bacillus aerius AV10 in solid-state fermentation of cellulosic wastes and it, s partial characterization. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 31(4 ), 0-0. SID. https://sid.ir/paper/366313/en

Vancouver: Copy

Azadian Kharanjani f., BADOEI DALFARD A., KARAMI Z.. Statistical optimization of Cellulase production by Bacillus aerius AV10 in solid-state fermentation of cellulosic wastes and it, s partial characterization. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;31(4 ):0-0. Available from: https://sid.ir/paper/366313/en

IEEE: Copy

f. Azadian Kharanjani, A. BADOEI DALFARD, and Z. KARAMI, “Statistical optimization of Cellulase production by Bacillus aerius AV10 in solid-state fermentation of cellulosic wastes and it, s partial characterization,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 31, no. 4 , pp. 0–0, 2019, [Online]. Available: https://sid.ir/paper/366313/en

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