DETECTION OF NIVALENOL SYNTHESIS GENE IN MADDER SEEDS INFECTED BY FUSARIUM SPECIES BY POLYMERASE CHAIN REACTION (PCR)

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HOSSEININEJAD SEYYED MOHSEN; ABEDI TIZAKI MUSTAFA; ESMAILZADEHHOSSEINI SEYYED ALIREZA; KARGAR FATEMEH; SADEGHI KHOMARTAJI KAMAL

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2017 | Volume:24 | Issue:12 Page(s): 952-962

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Title

DETECTION OF NIVALENOL SYNTHESIS GENE IN MADDER SEEDS INFECTED BY FUSARIUM SPECIES BY POLYMERASE CHAIN REACTION (PCR)

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Keywords

MADDERQ1

SEEDSQ1

FUSARIUMQ3

MYCOTOXINQ1

Abstract

Introduction: The MADDER is one of the most important crops. This product maybe infected by FUSARIUM species that produces potentially fatal MYCOTOXINs. The purpose of this current research was to identify trichothecene MYCOTOXINs produced by FUSARIUM fungi associated with MADDER SEEDS using molecular and biochemical methods.Methods: From different regions of Ardekan and Bafgh, sampling from MADDER SEEDS was done. Culture and purification of FUSARIUM isolates were taken place in specific media. Detection of fungi with the ability to produce trichothecenes MYCOTOXINs such as nivalenol (NIV) through gene-specific primers forTri13 by the polymerase chain reaction method (PCR) was performed. To confirm the NIV production potential, high performance liquid chromatography (HPLC) was applied.Results: In this study, five FUSARIUM species were identified from MADDER SEEDS. The results showed that among FUSARIUM species isolated from MADDER SEEDS, from which F. poae and F. equiseti had the ability to produce NIV. The gene involved in NIV synthesis, Tri13, was detected in two species, F. poae and F. equiseti, so that all these isolates were identified as NIV producing type. The HPLC performance showed that all studied FUSARIUM species had the potential to produce NIV MYCOTOXIN.Conclusion: Tri13 gene, in F. poae and F. equiseti, has a crucial role in trichothecene production. Thus, the PCR method can be used in various detections of MYCOTOXIN-producing fungi, which have the potential MYCOTOXIN production.

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APA: Copy

HOSSEININEJAD, SEYYED MOHSEN, ABEDI TIZAKI, MUSTAFA, ESMAILZADEHHOSSEINI, SEYYED ALIREZA, KARGAR, FATEMEH, & SADEGHI KHOMARTAJI, KAMAL. (2017). DETECTION OF NIVALENOL SYNTHESIS GENE IN MADDER SEEDS INFECTED BY FUSARIUM SPECIES BY POLYMERASE CHAIN REACTION (PCR). JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 24(12), 952-962. SID. https://sid.ir/paper/36677/en

Vancouver: Copy

HOSSEININEJAD SEYYED MOHSEN, ABEDI TIZAKI MUSTAFA, ESMAILZADEHHOSSEINI SEYYED ALIREZA, KARGAR FATEMEH, SADEGHI KHOMARTAJI KAMAL. DETECTION OF NIVALENOL SYNTHESIS GENE IN MADDER SEEDS INFECTED BY FUSARIUM SPECIES BY POLYMERASE CHAIN REACTION (PCR). JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;24(12):952-962. Available from: https://sid.ir/paper/36677/en

IEEE: Copy

SEYYED MOHSEN HOSSEININEJAD, MUSTAFA ABEDI TIZAKI, SEYYED ALIREZA ESMAILZADEHHOSSEINI, FATEMEH KARGAR, and KAMAL SADEGHI KHOMARTAJI, “DETECTION OF NIVALENOL SYNTHESIS GENE IN MADDER SEEDS INFECTED BY FUSARIUM SPECIES BY POLYMERASE CHAIN REACTION (PCR),” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 12, pp. 952–962, 2017, [Online]. Available: https://sid.ir/paper/36677/en

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