Genetic diversity of Staphylocoagulase genes (Coa) among methicillinresistant Staphylococcus aureus isolates at clinical specimens of blood and urinary infections

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

aghamohammadi mehrdad; FAGHRI JAMSHID

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2019 | Volume:77 | Issue:4 Page(s): 208-215

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

318

Download:

Cites:

Information Journal Paper

Title

Genetic diversity of Staphylocoagulase genes (Coa) among methicillinresistant Staphylococcus aureus isolates at clinical specimens of blood and urinary infections

Author(s)

aghamohammadi mehrdad | FAGHRI JAMSHID | Issue Writer Certificate

Keywords

coagulaseQ1

cross-sectional studiesQ1

genotypeQ2

polymerase chain reactionQ1

staphylococcus aureusQ2

Abstract

Background: staphylococcus aureus is a common pathogen in human that can be the cause of a wide range of infectious diseases including bacteremia, pneumonia, cellulitis, and osteomyelitis and skin and soft tissue infections. The coagulase enzyme is one of the most important virulence factors of this bacterium. The polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) Coa pattern is one of the molecular base typing methods. Molecular typing plays an important role in epidemiological studies of nosocomial infection, such as methicillin-resistant staphylococcus aureus (MRSA) infection. The PCR-RFLP Coa gene technique provides a useful preliminary method to monitor variations in MRSA populations. We were done Coa-RFLP typing according to the method of Hookey et al., with some modifications. Methods: In this cross-sectional study, one-hundred fifty isolates of S. aureus from urine and blood samples of patients that collected from educational hospitals of Imam Hossein and Al Zahra Isfahan University of Medical Sciences, Iran, from February 2018 to October 2018 were analyzed. After bacterial confirmation of isolates by Coa gene in polymerase chain reaction (PCR) technique, to perform coagulase gene typing, the repeated units encoding hypervariable regions of the coagulase gene of S. aureus were amplified by PCR. This was followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP) patterns. Results: Of 150 samples, 45 isolated of S. aureus were confirmed by biochemical methods. Of previous positive samples, 36 (80%) isolates carried Coa gene. Two different genotypes of Coa gene were obtained that include bp680 fragment in 20 specimens and bp750 fragment in 16 specimens. After enzymatic digestion by AluI restriction enzyme for RFLP, four different restriction patterns were obtained that including, the 280+400 pattern in 16 specimens (44. 4%), 280+470 pattern in 7 specimens (19. 4%), 340+340 pattern in 6 specimens (16. 6%) and 750 patterns without digestion were in 7 specimens (19. 6%). Conclusion: Using the present experiments, it was determined that the PCR-RFLP pattern, 280+400, was the dominant pattern in the staphylococcus aureus samples isolated in Isfahan.

Cites

No record.

References

Cite

APA: Copy

aghamohammadi, mehrdad, & FAGHRI, JAMSHID. (2019). Genetic diversity of Staphylocoagulase genes (Coa) among methicillinresistant Staphylococcus aureus isolates at clinical specimens of blood and urinary infections. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 77(4 ), 208-215. SID. https://sid.ir/paper/366808/en

Vancouver: Copy

aghamohammadi mehrdad, FAGHRI JAMSHID. Genetic diversity of Staphylocoagulase genes (Coa) among methicillinresistant Staphylococcus aureus isolates at clinical specimens of blood and urinary infections. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2019;77(4 ):208-215. Available from: https://sid.ir/paper/366808/en

IEEE: Copy

mehrdad aghamohammadi, and JAMSHID FAGHRI, “Genetic diversity of Staphylocoagulase genes (Coa) among methicillinresistant Staphylococcus aureus isolates at clinical specimens of blood and urinary infections,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 77, no. 4 , pp. 208–215, 2019, [Online]. Available: https://sid.ir/paper/366808/en

Related Journal Papers