ISOLATION OF PNEUMOCYSTIS JIROVECII FROM IMMUNOCOMPETENT AND IMMUNODEFICIENT PATIENTS WITH PULMONARY DISORDER (A MOLECULAR STUDY)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ABDOLLAHI B.; REZAEITALAB F.; FATA A.; ATTARAN D.; NAJAFZADEH M.J.; VAKILI V.; PARIAN M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Medical Journal of Mashhad University of Medical Sciences Year:2017 | Volume:59 | Issue:6 Page(s): 272-282

Download Full-Text

Persian Verion

View:

413

Download:

Cites:

Information Journal Paper

Title

ISOLATION OF PNEUMOCYSTIS JIROVECII FROM IMMUNOCOMPETENT AND IMMUNODEFICIENT PATIENTS WITH PULMONARY DISORDER (A MOLECULAR STUDY)

Author(s)

Keywords

NEUMOCYSTIS JIROVECII‚Q1

BRONCHOALVEOLAR LAVAGE (BAL) ‚Q1

POLYMERASE CHAIN REACTION (PCR) ‚Q2

PNEUMOCYSTIS PNEUMONIA (PCP)Q1

Abstract

Introduction: Pneumocystis pneumonia (PCP) is one of the most serious life threatening infections in patients with human immunodeficiency virus. It can also occur in immunosuppressed patients treated with immunosuppressive drugs. Immunocompetent adults can carryPneumocystis. The aim of this study was to identify Pneumocystis jirovecii in patients with pulmonary disorder. Materials and Methods: During 9 months (September 2014-May 2015), 138 Bronchoalveolar Lavage (BAL) samples obtained from patients undergone bronchoscopy referred to different departments of University Hospitals of Mashhad. Giemsa stained smear and molecular method performed for each sample. After DNA extraction, amplification of mitochondrial gene coding ribosomal large subunit (mtLSU-rRNA) ofP. jirovecii was performed by Nested-PCR. To reconfirm diagnosis and definite identification, gene sequencing was performed. For statistical analysis t-test and chi-square tests were used, by using SPSS version 16. Results: By direct microscopy, P. jirovecii was diagnosed in only 3(2. 2%) samples, but DNA was detected in 17 (12. 3%) by nested PCR. P. jirovecii isolated from 10 (25%) immunosuppressed and 7 (7. 1%) of immunocompetent individuals. Fever, dyspnea and dry cough were the most common symptoms among patients with P. jirovecii. Conclusion: P. jirovecii is prevalent in immunodeficient patients compared to immunocompetent individuals. The latter group can act as reservoir of P. jirovecii and can be an important source of infection for patients with pulmonary disorders.

Cites

No record.

References

No record.

Cite

APA: Copy

ABDOLLAHI, B., REZAEITALAB, F., FATA, A., ATTARAN, D., NAJAFZADEH, M.J., VAKILI, V., & PARIAN, M.. (2017). ISOLATION OF PNEUMOCYSTIS JIROVECII FROM IMMUNOCOMPETENT AND IMMUNODEFICIENT PATIENTS WITH PULMONARY DISORDER (A MOLECULAR STUDY). MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, 59(6 ), 272-282. SID. https://sid.ir/paper/366838/en

Vancouver: Copy

ABDOLLAHI B., REZAEITALAB F., FATA A., ATTARAN D., NAJAFZADEH M.J., VAKILI V., PARIAN M.. ISOLATION OF PNEUMOCYSTIS JIROVECII FROM IMMUNOCOMPETENT AND IMMUNODEFICIENT PATIENTS WITH PULMONARY DISORDER (A MOLECULAR STUDY). MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;59(6 ):272-282. Available from: https://sid.ir/paper/366838/en

IEEE: Copy

B. ABDOLLAHI, F. REZAEITALAB, A. FATA, D. ATTARAN, M.J. NAJAFZADEH, V. VAKILI, and M. PARIAN, “ISOLATION OF PNEUMOCYSTIS JIROVECII FROM IMMUNOCOMPETENT AND IMMUNODEFICIENT PATIENTS WITH PULMONARY DISORDER (A MOLECULAR STUDY),” MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, vol. 59, no. 6 , pp. 272–282, 2017, [Online]. Available: https://sid.ir/paper/366838/en

Related Journal Papers