Long-term storage of Buxus hyrcana Pojark. gerplasm, an ornamental shrub in danger of extinction, under cryopreservation conditions with encapsulation dehydration and its regeneration by phytohormones

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KAVIANI BEHZAD; NEGAHDAR NASER

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Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2018 | Volume:7 | Issue:23 Page(s): 337-346

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Title

Long-term storage of Buxus hyrcana Pojark. gerplasm, an ornamental shrub in danger of extinction, under cryopreservation conditions with encapsulation dehydration and its regeneration by phytohormones

Author(s)

KAVIANI BEHZAD | NEGAHDAR NASER | Issue Writer Certificate

Keywords

Liquid nitrogenQ1

Gene bankQ1

Synthetic seedQ2

Genetic poolQ1

Ornamental plantsQ2

Abstract

Box tree (Buxus sempervirens L. or Buxus hyrcana Pojark. ), is an ornamental shrub species that has applications in various industries such as handmade and ornamental industeries. This species is in danger of extinction. Conservation of plants germplasm especially the plants in danger of extinction is one of the purposes of researchers and parlements members all of the worlds. Thus, the aim of this research was long-term conservation of germplasm in Liquid nitrogen with sucrose and encapsulation-dehydration pre-treatments. Used germplasms or explants were seed and apical buds which were prepared from mother plants grown in greenhouse. This research presents a suitable method for sterilization of explants especially apical buds. Concentrations of 0, 0. 5, 1, 1. 5 and 2 mg l-1 of three plant growth regulators BAP, IBA and NAA were used in germplasm regeneration medium after conservation in Liquid nitrogen. The experiment was carried out as factorial based on a randomized complete block design in four replications. The results of the research showed that encapsulation as a pre-treatment had effective role on the survival and germination of apical buds. Around 50% of encapsulated apical buds were attained their germination capacity. The highest germination percentage of encapsulated apical buds (60%) was obtained in culture medium containing 0. 5 mg l-1 BAP along with 1. 5 mg l-1 NAA. Medium containing 0. 5 mg l-1 BAP without NAA with the content of 48% germination induction of apical buds was a suitable medium, too. None of non-encapsulated apical buds and encapsulated and non-encapsulated seeds had survival after conservation in Liquid nitrogen and cultivation in regeneration medium.

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APA: Copy

KAVIANI, BEHZAD, & NEGAHDAR, NASER. (2018). Long-term storage of Buxus hyrcana Pojark. gerplasm, an ornamental shrub in danger of extinction, under cryopreservation conditions with encapsulation dehydration and its regeneration by phytohormones. JOURNAL OF PLANT PROCESS AND FUNCTION, 7(23 ), 337-346. SID. https://sid.ir/paper/368101/en

Vancouver: Copy

KAVIANI BEHZAD, NEGAHDAR NASER. Long-term storage of Buxus hyrcana Pojark. gerplasm, an ornamental shrub in danger of extinction, under cryopreservation conditions with encapsulation dehydration and its regeneration by phytohormones. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2018;7(23 ):337-346. Available from: https://sid.ir/paper/368101/en

IEEE: Copy

BEHZAD KAVIANI, and NASER NEGAHDAR, “Long-term storage of Buxus hyrcana Pojark. gerplasm, an ornamental shrub in danger of extinction, under cryopreservation conditions with encapsulation dehydration and its regeneration by phytohormones,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 7, no. 23 , pp. 337–346, 2018, [Online]. Available: https://sid.ir/paper/368101/en

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