ISOLATION, CLONING AND ANALYSIS OF THE GLUCOSE TRANSPORTER GENE 7 FROM IRANIAN STRAIN OF SACCHAROMYCES CEREVISIAE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZIZI SOLMAZ; TARINEJAD ALIREZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Koomesh Year:2015 | Volume:16 | Issue:3 (55) Page(s): 356-365

Download Full-Text

Persian Verion

View:

855

Download:

Cites:

Information Journal Paper

Title

ISOLATION, CLONING AND ANALYSIS OF THE GLUCOSE TRANSPORTER GENE 7 FROM IRANIAN STRAIN OF SACCHAROMYCES CEREVISIAE

Author(s)

AZIZI SOLMAZ | TARINEJAD ALIREZA | Issue Writer Certificate

Keywords

CLONINGQ2

SACCHAROMYCES CEREVISIAEQ1

HXT7 GENEQ2

RECOMBINANT PLASMIDSQ2

Abstract

Introduction: The most important and specific known action for SACCHAROMYCES CEREVISIAE yeast is producing ethanol by alcohol fermentation and that is probably due to its high efficiency for absorption and fermentation of hexose sugars. The S. cerevisiae express 20 genes that encode hexose transporter proteins, including hxt1-hxt17, gal2, snf3 and rgt2. Among all those gene families, hxt1-hxt7 have important role in alcohol production. It has been shown that increasing the expression hxt1-hxt7 accelerates alcohol fermentation and therefore, ethanol production. The aim of this study was to identify and isolate hxt7 from SACCHAROMYCES CEREVISIAE genome, using PCR and CLONING it into a vector containing suitable expression promoter in order to produce recombinant yeast by transformation.Materials and Methods: Isolation of hxt7 by specific primers was achieved via PCR. The amplified fragments were cloned into pGEM-T vector and transformed into Escherichia coli and finally, the RECOMBINANT PLASMIDS were sent to sequencing.Results: The nucleotide sequence of open reading frame in gene was revealed a 1713 bp long with a deduced amino acid of 570 residues. The estimated molecular mass and the predicted isoelectric point of the deduced polypeptide were 62.725 kDa and 7.89, respectively.Conclusion: The deduced protein sequence showed a high similarity to hxt7 sequences registered in NCBI and with the highest percentage of similarity to Hxt7p S. cerevisiae S288C recorded at NCBI with access code NP010629.3.

Cites

No record.

References

No record.

Cite

APA: Copy

AZIZI, SOLMAZ, & TARINEJAD, ALIREZA. (2015). ISOLATION, CLONING AND ANALYSIS OF THE GLUCOSE TRANSPORTER GENE 7 FROM IRANIAN STRAIN OF SACCHAROMYCES CEREVISIAE. KOOMESH, 16(3 (55)), 356-365. SID. https://sid.ir/paper/36819/en

Vancouver: Copy

AZIZI SOLMAZ, TARINEJAD ALIREZA. ISOLATION, CLONING AND ANALYSIS OF THE GLUCOSE TRANSPORTER GENE 7 FROM IRANIAN STRAIN OF SACCHAROMYCES CEREVISIAE. KOOMESH[Internet]. 2015;16(3 (55)):356-365. Available from: https://sid.ir/paper/36819/en

IEEE: Copy

SOLMAZ AZIZI, and ALIREZA TARINEJAD, “ISOLATION, CLONING AND ANALYSIS OF THE GLUCOSE TRANSPORTER GENE 7 FROM IRANIAN STRAIN OF SACCHAROMYCES CEREVISIAE,” KOOMESH, vol. 16, no. 3 (55), pp. 356–365, 2015, [Online]. Available: https://sid.ir/paper/36819/en

Related Journal Papers