INDUCTION OF RESPIRATORY BURST IN HUMAN MONOCYTES AFTER TREATING WITH PLATELET-DERIVED MICROPARTICLES

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KOUSHKI SAMANEH; YARI FATEMEH; SHAIEGAN MOJGAN

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Paper Information

Journal: Koomesh Year:2016 | Volume:17 | Issue:4 (60) Page(s): 1033-1039

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Information Journal Paper

Title

INDUCTION OF RESPIRATORY BURST IN HUMAN MONOCYTES AFTER TREATING WITH PLATELET-DERIVED MICROPARTICLES

Author(s)

KOUSHKI SAMANEH | YARI FATEMEH | SHAIEGAN MOJGAN | Issue Writer Certificate

Keywords

BLOOD PLATELETSQ2

CELL-DERIVED MICROPARTICLESQ1

MONOCYTESQ2

RESPIRATORY BURSTQ3

Abstract

Introduction: Activated platelets in platelet concentrate, release membranous vesicles that called platelet microparticles and can be created spontaneously during the storage of platelets. Studies have shown that platelet microparticles can form aggregates with MONOCYTES so we intended to survey the ability of these microvesicles to activate MONOCYTES.Materials and Methods: Platelet concentrate bags (PCs) were prepared from Iranian Blood Transfusion Organization in Tehran. Sampling of the bags and isolation of PMP was carried out during storage. Besides, the RESPIRATORY BURST of MONOCYTES was measured by flow cytometry technique before and after treatment with PMP using di-hydrorhodamine-123 (DHR-123(.Results: Monocyte cells derived from human peripheral blood were activated after exposure to platelet microparticles and intracellular RESPIRATORY BURST was significantly increased (P-value=0.01). These findings suggest that PMPs have a dose-dependent effect on MONOCYTES and they are more effective at the concentration of 500 micrograms per milliliter.Conclusion: This study showed the potential of platelet microparticles in the stimulation and activation of human peripheral blood MONOCYTES.

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References

USING SIX-COLOUR FLOW CYTOMETRY TO ANALYSE THE ACTIVATION AND INTERACTION OF PLATELETS AND LEUKOCYTES-A NEW ASSAY SUITABLE FOR BENCH AND BEDSIDE CONDITIONS

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APA: Copy

KOUSHKI, SAMANEH, YARI, FATEMEH, & SHAIEGAN, MOJGAN. (2016). INDUCTION OF RESPIRATORY BURST IN HUMAN MONOCYTES AFTER TREATING WITH PLATELET-DERIVED MICROPARTICLES. KOOMESH, 17(4 (60)), 1033-1039. SID. https://sid.ir/paper/36905/en

Vancouver: Copy

KOUSHKI SAMANEH, YARI FATEMEH, SHAIEGAN MOJGAN. INDUCTION OF RESPIRATORY BURST IN HUMAN MONOCYTES AFTER TREATING WITH PLATELET-DERIVED MICROPARTICLES. KOOMESH[Internet]. 2016;17(4 (60)):1033-1039. Available from: https://sid.ir/paper/36905/en

IEEE: Copy

SAMANEH KOUSHKI, FATEMEH YARI, and MOJGAN SHAIEGAN, “INDUCTION OF RESPIRATORY BURST IN HUMAN MONOCYTES AFTER TREATING WITH PLATELET-DERIVED MICROPARTICLES,” KOOMESH, vol. 17, no. 4 (60), pp. 1033–1039, 2016, [Online]. Available: https://sid.ir/paper/36905/en

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