The effect of growth regulators, sucrose density and cold treatment on proliferation, cormlet produce and breaking dormancy in two gladiolus cultivars

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Kalantari Ashkaneh; KOOBAZ PARISA; ROSTAMI MAJID; Fathi Ghare baba Mohammad; MOJTAHEDI NARGES

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Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2019 | Volume:8 | Issue:33 Page(s): 203-211

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Title

The effect of growth regulators, sucrose density and cold treatment on proliferation, cormlet produce and breaking dormancy in two gladiolus cultivars

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Keywords

Cold stratificationQ1

CormletQ1

DormancyQ2

Micro-propagationQ1

Abstract

Enhancement of in vitro micro corm production in Gladiolus as an alternative commercial method has been focus of attention. With respet to the position of Gladiolus in Iran's ornamental plants market and in order to provide the proper protocol for the proliferation of glycol corms by tissue culture method, the effect of different concentrations of naphthalene acetic acid (NAA) and benzyl aminopurine (BAP) in two explants and production of shoot and roots of two commercial gladiolus cultivars were investigated. In the next experiment the role of different concentrations of sucrose (in Murashige and Skoog plant growth liquid medium) in production of corms was studied and finally cold treatment in 3 times were used to break Dormancy of propagated Cormlets. All of the main experiments were conducted as factorial based on completely randomized design with 3 replications. The highest number of shoots (14) and roots (12) was observed in White cultivar with the treatments of 2mg/L BAP and 0. 5 mg/ml NAA. Application of 60 g/L sucrose in MS media produced the highest number of Cormlets (14) and also the largest Cormlets (14. 5 mm). Results of Dormancy breaking experiment which performed at 4 ° C for three periods showed that three months chilling at 4 ° C was the best treatment to break Dormancy in both cultivars.

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APA: Copy

Kalantari, Ashkaneh, KOOBAZ, PARISA, ROSTAMI, MAJID, Fathi Ghare baba, Mohammad, & MOJTAHEDI, NARGES. (2019). The effect of growth regulators, sucrose density and cold treatment on proliferation, cormlet produce and breaking dormancy in two gladiolus cultivars. JOURNAL OF PLANT PROCESS AND FUNCTION, 8(33 ), 203-211. SID. https://sid.ir/paper/369259/en

Vancouver: Copy

Kalantari Ashkaneh, KOOBAZ PARISA, ROSTAMI MAJID, Fathi Ghare baba Mohammad, MOJTAHEDI NARGES. The effect of growth regulators, sucrose density and cold treatment on proliferation, cormlet produce and breaking dormancy in two gladiolus cultivars. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2019;8(33 ):203-211. Available from: https://sid.ir/paper/369259/en

IEEE: Copy

Ashkaneh Kalantari, PARISA KOOBAZ, MAJID ROSTAMI, Mohammad Fathi Ghare baba, and NARGES MOJTAHEDI, “The effect of growth regulators, sucrose density and cold treatment on proliferation, cormlet produce and breaking dormancy in two gladiolus cultivars,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 8, no. 33 , pp. 203–211, 2019, [Online]. Available: https://sid.ir/paper/369259/en

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