DESIGN OF ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD FOR DETECTION OF ANTI-STREPTOLYSIN-O ANTIBODIES ON BASE OF RECOMBINANT STREPTOLYSIN-O

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MOSAYEBI GHASEM; ABTAHI HAMEID; GHAZAVI ALI; ZAREINFAR NADER; KHAKI MOHSEN; PAYANI MOHAMMAD ALI

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Journal: Koomesh Year:2012 | Volume:13 | Issue:3 (43) Page(s): 362-367

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Title

DESIGN OF ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD FOR DETECTION OF ANTI-STREPTOLYSIN-O ANTIBODIES ON BASE OF RECOMBINANT STREPTOLYSIN-O

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Keywords

STREPTOCOCCI PYOGENEQ2

RECOMBINANT STREPTOLYSIN-OQ2

ANTI-STREPTOLYSIN-O ANTIBODIESQ2

ENZYMELINKED IMMUNOSORBENT ASSAYQ2

Abstract

Introduction: Group A, b hemolytic streptococci are among the major causative agents of otorhinolaryngology infections. Inadequate treatment of disease may lead to serious disorders such as acute rheumatic fever and glomerulonephritis. Anti-streptolysin-O (ASO) is commonly used as a marker in the diagnosis of infection. Purification of native streptolysin-O has several difficulties and its industrial production process is time consuming with very low yield and the risk of biological contamination. In this study, we used a RECOMBINANT STREPTOLYSIN-O protein as an antigen to detect ASO antibodies in enzymeslinked immunosorbent assay (ELISA).Materials and Methods: We amplified streptolysin-O gene by polymerase chain reaction (PCR) method and subcloned in prokaryotic expression vector PET28a. E.coli. BL21-DE3-plySs strain was transformed with PET28a-streptolysin-O and gene expression was induced by IPTG. ELISA microplates were coated with different concentration of streptolysin-O protein. Level of ASO antibodies were detected by ELISA method. The results obtained from ELISA method were compared with inhibition of hemolysis assay as a standard method.Results: The results showed that there is a positive significant correlation between the ELISA and inhibition of hemolysis method (r=0.97, p=0.0001). The sensitivity and specificity of ELISA for detection of ASO anti bodies were 100% and 83%, respectively.Conclusion: ELISA developed with recombinant streptolysine O showed a good sensitivity for detection of ASO antibodies. It is suggested that this method could be suitable for immunoassays.

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APA: Copy

MOSAYEBI, GHASEM, ABTAHI, HAMEID, GHAZAVI, ALI, ZAREINFAR, NADER, KHAKI, MOHSEN, & PAYANI, MOHAMMAD ALI. (2012). DESIGN OF ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD FOR DETECTION OF ANTI-STREPTOLYSIN-O ANTIBODIES ON BASE OF RECOMBINANT STREPTOLYSIN-O. KOOMESH, 13(3 (43)), 362-367. SID. https://sid.ir/paper/36947/en

Vancouver: Copy

MOSAYEBI GHASEM, ABTAHI HAMEID, GHAZAVI ALI, ZAREINFAR NADER, KHAKI MOHSEN, PAYANI MOHAMMAD ALI. DESIGN OF ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD FOR DETECTION OF ANTI-STREPTOLYSIN-O ANTIBODIES ON BASE OF RECOMBINANT STREPTOLYSIN-O. KOOMESH[Internet]. 2012;13(3 (43)):362-367. Available from: https://sid.ir/paper/36947/en

IEEE: Copy

GHASEM MOSAYEBI, HAMEID ABTAHI, ALI GHAZAVI, NADER ZAREINFAR, MOHSEN KHAKI, and MOHAMMAD ALI PAYANI, “DESIGN OF ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD FOR DETECTION OF ANTI-STREPTOLYSIN-O ANTIBODIES ON BASE OF RECOMBINANT STREPTOLYSIN-O,” KOOMESH, vol. 13, no. 3 (43), pp. 362–367, 2012, [Online]. Available: https://sid.ir/paper/36947/en

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