Effects of Phagocytosis of Apoptotic Wharton Jelly Mesenchymal Stem Cells on the Activity of C57BL/6 Mice Peritoneal Macrophages

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Ghahremani Piraghaj Maryam; HASHEMI SEYED MAHMOUD; Ghanbarian Hossein; Masoudi Sara

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Journal: Research in Medicine Year:2018 | Volume:42 | Issue:3 Page(s): 160-166

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Title

Effects of Phagocytosis of Apoptotic Wharton Jelly Mesenchymal Stem Cells on the Activity of C57BL/6 Mice Peritoneal Macrophages

Author(s)

Ghahremani Piraghaj Maryam | HASHEMI SEYED MAHMOUD | Ghanbarian Hossein | Masoudi Sara | Issue Writer Certificate

Keywords

MacrophageQ2

Wharton's jellyQ1

Mesenchymal Stem CellQ1

EfferocytosisQ3

PhagocytosisQ3

Abstract

Background: Macrophages are one of the most important immune cells. They can be divided into two main subgroups of classical or inflammatory Macrophages (M1) and alternative or non-inflammatory Macrophages (M2), due to different stimuli. One of the factors that make the Macrophage to orient towards M2 is the Phagocytosis of apoptosis cells (Efferocytosis). The Phagocytosis of Mesenchymal Stem Cells can be very important in cell therapy due to their immunomedulatory properties and the ability to modulate Macrophage function. Materials and Methods: Mesenchymal Stem Cells were isolated from Wharton’ s jelly and characterized using flow cytometry as well as differentiation to Osteoblasts and adipocytes. MSCs in passage two were exposed to UV light for induction of apoptosis for 30 minutes followed by incubation for two hours. The cells were then isolated and added to Macrophages in a ratio of 4 to 1. Next, cells were incubated for 48 hours, and then the productions of TNFα and IL10 cytokines were measured using ELISA and nitric oxide production was measured using Griess method. Phagocytosis ability of the Macrophages was also measured using yeast and apoptotic thymocytes Phagocytosis in different groups. Results: The Phagocytosis of Wharton’ s jelly-Mesenchymal Stem Cells (WJ– MSCs) by Macrophages reduces the production of inflammatory cytokine TNFα and increases the production of inhibitory cytokine IL-10. Nitric oxide production decreased in these Macrophages. In addition, after Phagocytosis of apoptotic WJ-MSCs, the ability of yeast Phagocytosis in these Macrophages was reduced and Phagocytosis of apoptotic thymocyte was increased. Conclusion: The Phagocytosis of apoptotic WJ-MSCs induces non-inflammatory phenotype in Macrophages. Therefore, injected WJ-MSCs maintain their immunomodulatory properties even if they get apoptosed in the body.

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APA: Copy

Ghahremani Piraghaj, Maryam, HASHEMI, SEYED MAHMOUD, Ghanbarian, Hossein, & Masoudi, Sara. (2018). Effects of Phagocytosis of Apoptotic Wharton Jelly Mesenchymal Stem Cells on the Activity of C57BL/6 Mice Peritoneal Macrophages. PAJOUHESH DAR PEZESHKI, 42(3 ), 160-166. SID. https://sid.ir/paper/370751/en

Vancouver: Copy

Ghahremani Piraghaj Maryam, HASHEMI SEYED MAHMOUD, Ghanbarian Hossein, Masoudi Sara. Effects of Phagocytosis of Apoptotic Wharton Jelly Mesenchymal Stem Cells on the Activity of C57BL/6 Mice Peritoneal Macrophages. PAJOUHESH DAR PEZESHKI[Internet]. 2018;42(3 ):160-166. Available from: https://sid.ir/paper/370751/en

IEEE: Copy

Maryam Ghahremani Piraghaj, SEYED MAHMOUD HASHEMI, Hossein Ghanbarian, and Sara Masoudi, “Effects of Phagocytosis of Apoptotic Wharton Jelly Mesenchymal Stem Cells on the Activity of C57BL/6 Mice Peritoneal Macrophages,” PAJOUHESH DAR PEZESHKI, vol. 42, no. 3 , pp. 160–166, 2018, [Online]. Available: https://sid.ir/paper/370751/en

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