DESIGN AND CONSTRUCTION OF FUSION GENES HSPX AND TB10.4 FROM MYCOBACTERIUM TUBERCULOSIS IN A CLONING VECTOR

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Yaghoubi Atieh; ARYAN EHSAN; DERAKHSHAN MOHAMMAD; MESHKAT ZAHRA

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Journal: Koomesh Year:2017 | Volume:19 | Issue:1 (65) Page(s): 183-190

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Title

DESIGN AND CONSTRUCTION OF FUSION GENES HSPX AND TB10.4 FROM MYCOBACTERIUM TUBERCULOSIS IN A CLONING VECTOR

Author(s)

Yaghoubi Atieh | ARYAN EHSAN | DERAKHSHAN MOHAMMAD | MESHKAT ZAHRA | Issue Writer Certificate

Keywords

MYCOBACTERIUM TUBERCULOSISQ2

CLONINGQ2

TB10.4Q1

HSPXQ1

DNA VACCINEQ2

Abstract

Introduction: Tuberculosis (TB) is the most important infectious disease and is one of the most common causes of death in the world especially in developing countries. TB is caused by infection with MYCOBACTERIUM TUBERCULOSIS. Designing and construction new vaccines against MYCOBACTERIUM TUBERCULOSIS are the only effective way to prevent and control the disease. The aim of this study was to design and construct a CLONING vector encoding HSPX and TB10.4 fusion genes of MYCOBACTERIUM TUBERCULOSIS.Materials and Methods: At first, TB10.4 fragment was amplified by PCR method and it was digested with restriction enzymes and was cloned into the plasmid pcDNA3.1 +. Then, HSPX fragment was amplified by PCR method and it was digested by HindIII and BamHI restriction enzymes. The recombinant plasmid pcDNA3.1 + / TB10.4 also digested with the same enzymes and HSPX was subcloned into the recombinant vector. This construct was transformed into the Escherichia coli strain TOP10. The confirming the clones were performed by colony PCR, restriction enzyme digestion and sequencing methods.Results: PCR and enzymatic digestion products were run on the agarose gel and TB10.4 and HSPX genes were observed 291bp and 435bp, respectively. In addition, results of DNA sequencing showed 100% homology with HSPX and TB10.4 genes of MYCOBACTERIUM TUBERCULOSIS H37Rv strain recorded in GenBank.Conclusion: In this study, HSPX and TB10.4 genes of MYCOBACTERIUM TUBERCULOSIS were cloned into pcDNA3.1 + vector correctly. This vector can use as a DNA VACCINE to induce immune system responses in animal models in future studies.

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APA: Copy

Yaghoubi, Atieh, ARYAN, EHSAN, DERAKHSHAN, MOHAMMAD, & MESHKAT, ZAHRA. (2017). DESIGN AND CONSTRUCTION OF FUSION GENES HSPX AND TB10.4 FROM MYCOBACTERIUM TUBERCULOSIS IN A CLONING VECTOR. KOOMESH, 19(1 (65)), 183-190. SID. https://sid.ir/paper/37109/en

Vancouver: Copy

Yaghoubi Atieh, ARYAN EHSAN, DERAKHSHAN MOHAMMAD, MESHKAT ZAHRA. DESIGN AND CONSTRUCTION OF FUSION GENES HSPX AND TB10.4 FROM MYCOBACTERIUM TUBERCULOSIS IN A CLONING VECTOR. KOOMESH[Internet]. 2017;19(1 (65)):183-190. Available from: https://sid.ir/paper/37109/en

IEEE: Copy

Atieh Yaghoubi, EHSAN ARYAN, MOHAMMAD DERAKHSHAN, and ZAHRA MESHKAT, “DESIGN AND CONSTRUCTION OF FUSION GENES HSPX AND TB10.4 FROM MYCOBACTERIUM TUBERCULOSIS IN A CLONING VECTOR,” KOOMESH, vol. 19, no. 1 (65), pp. 183–190, 2017, [Online]. Available: https://sid.ir/paper/37109/en

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