Inhibitory effect of the effective fraction of Gundelia tournefortii L and several anti-diabetic drugs on the aldose reductase activity isolated from cow lens eye: a comparative study

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Asareh Z.; BAHRAMIKIA S.

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Journal: Journal of Animal Research Year:2019 | Volume:32 | Issue:3 Page(s): 232-245

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Title

Inhibitory effect of the effective fraction of Gundelia tournefortii L and several anti-diabetic drugs on the aldose reductase activity isolated from cow lens eye: a comparative study

Author(s)

Asareh Z. | BAHRAMIKIA S. | Issue Writer Certificate

Keywords

polyol pathwayQ1

aldose reductaseQ1

chronic diabetes complicationsQ1

Gundela tournefortiiQ1

ethyl acetate fractionQ1

Abstract

Diabetes mellitus in many countries among adults aged 20-70 is the main cause of blindness, amputation and chronic renal failure. Increasing blood glucose and entering glucose into the polyol pathway, is one of the factors involved in the pathogenesis of chronic diabetes complications. Since the key enzyme in this pathway is aldose reductase, the use of plant or chemical compounds that reduce the activity of this enzyme can be used as a therapeutic strategy to prevent the onset of chronic complications of diabetes. In this study, the effect of Gundela tournefortii crude extract and its different fractions in Lorestan province and also several anti-diabetes drugs on inhibition of aldose reductase enzyme were investigated. For this purpose, firstly, the phenolic and flavonoid content and antioxidant activity of crude extract and various fractions were determined. In the next step, the cow lenses were extracted, the aldose reductase enzyme was isolated by Hayman-Kinoshita method and the inhibitory effect of the compounds on the activity of the enzyme was determined. Then, using the kinetics of the enzyme, the type of inhibition of the ethyl acetate fraction was determined. The results showed that the ethyl acetate fraction has the highest amount of phenol and flavonoid compounds and the highest capacity for the DPPH radical scavenging. Also, this fraction has the highest inhibitory effect on aldose reductase enzyme activity with IC50 = 0. 019 mg/ml. This fraction had uncompetitive inhibition with D-glyceraldehyde, but it showed competitive inhibition to NADPH. Anti-diabetic drugs have been shown do not significantly affect the inhibition of the enzyme and are likely to affect other mechanisms of diabetes.

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APA: Copy

Asareh, Z., & BAHRAMIKIA, S.. (2019). Inhibitory effect of the effective fraction of Gundelia tournefortii L and several anti-diabetic drugs on the aldose reductase activity isolated from cow lens eye: a comparative study. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(3 ), 232-245. SID. https://sid.ir/paper/371816/en

Vancouver: Copy

Asareh Z., BAHRAMIKIA S.. Inhibitory effect of the effective fraction of Gundelia tournefortii L and several anti-diabetic drugs on the aldose reductase activity isolated from cow lens eye: a comparative study. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(3 ):232-245. Available from: https://sid.ir/paper/371816/en

IEEE: Copy

Z. Asareh, and S. BAHRAMIKIA, “Inhibitory effect of the effective fraction of Gundelia tournefortii L and several anti-diabetic drugs on the aldose reductase activity isolated from cow lens eye: a comparative study,” JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 3 , pp. 232–245, 2019, [Online]. Available: https://sid.ir/paper/371816/en

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