EFFECT OF SOLUBLE CARCINOEMBRYONIC ANTIGEN ON MYOGENIC AND ENTEROCYTIC DIFFERENTIATION

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PAKDEL ABBAS; NAGHIBALHOSSAINI FAKHRADDIN

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Journal: Koomesh Year:2013 | Volume:14 | Issue:3 (47) Page(s): 373-381

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Information Journal Paper

Title

EFFECT OF SOLUBLE CARCINOEMBRYONIC ANTIGEN ON MYOGENIC AND ENTEROCYTIC DIFFERENTIATION

Author(s)

PAKDEL ABBAS | NAGHIBALHOSSAINI FAKHRADDIN | Issue Writer Certificate

Keywords

CARCINOEMBRYONIC ANTIGENQ2

COLORECTAL NEOPLASMSQ2

MYOGENINQ2

Abstract

Introduction: CARCINOEMBRYONIC ANTIGEN (CEA), a glycophosphatidyl inositol (GPI) anchored glycoprotein, is over-expressed in various cancers, including colorectal carcinomas. Inhibition of cell differentiation can cause cancer. Previous studies show transfection and over-expression of CEA gene in myoblasts or colonocytes leads to inhibition of cell differentiation. We investigated whether soluble CEA has a role in inhibition of cell differentiation.Materials and Methods: Monolayer cultures of L6, C2C12, Caco-2 and CHO cell lines were grown in dulbecco's modification of eagle's medium (DMEM) containing 10% fetal bovine serum (FBS). For differentiation induction C2C12 and L6 cell lines media, was changed to DMEM containing 2% horse serum. CEA concentration was measured in harvested media by ELISA assay. H&E staining, CK assay and RT-PCR for MYOGENIN gene were used for C2C12 differentiation experiments.Results: The results showed although commercial pure CEA and LS-180 conditioned media (contain high level of CEA) have inhibitory effects on Caco-2 differentiation, but it can also inhibit differentiation of control groups. We observed the effect of high concentration of CEA (5mg/ml) on L6 differentiation is identical with the effect of BSA as control. C2C12 differentiation was inhibited in response to LS-180 conditioned media at morphological level and MYOGENIN expression. CK activity was significantly lower in LS-180 conditioned media (P=0.0012) and CHO (P=0.0002) conditioned media groups in comparison to control group.Conclusion: Our finding shows soluble CEA antigen did not have a significant effect on cell differentiation.

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APA: Copy

PAKDEL, ABBAS, & NAGHIBALHOSSAINI, FAKHRADDIN. (2013). EFFECT OF SOLUBLE CARCINOEMBRYONIC ANTIGEN ON MYOGENIC AND ENTEROCYTIC DIFFERENTIATION. KOOMESH, 14(3 (47)), 373-381. SID. https://sid.ir/paper/37249/en

Vancouver: Copy

PAKDEL ABBAS, NAGHIBALHOSSAINI FAKHRADDIN. EFFECT OF SOLUBLE CARCINOEMBRYONIC ANTIGEN ON MYOGENIC AND ENTEROCYTIC DIFFERENTIATION. KOOMESH[Internet]. 2013;14(3 (47)):373-381. Available from: https://sid.ir/paper/37249/en

IEEE: Copy

ABBAS PAKDEL, and FAKHRADDIN NAGHIBALHOSSAINI, “EFFECT OF SOLUBLE CARCINOEMBRYONIC ANTIGEN ON MYOGENIC AND ENTEROCYTIC DIFFERENTIATION,” KOOMESH, vol. 14, no. 3 (47), pp. 373–381, 2013, [Online]. Available: https://sid.ir/paper/37249/en

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