Effect of loratadine on YKL-40 production in Mite extract stimulated A549 and THP-1 cells

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Baqernezhadan Zeinab; Darbandi Tamijani Hasan; Hajimollahoseini Mostafa

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Journal: Research in Medicine Year:2019 | Volume:42 | Issue:4 Page(s): 202-208

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Title

Effect of loratadine on YKL-40 production in Mite extract stimulated A549 and THP-1 cells

Author(s)

Baqernezhadan Zeinab | Darbandi Tamijani Hasan | Hajimollahoseini Mostafa | Issue Writer Certificate

Keywords

AllergyQ3

LoratadineQ1

YKL-40Q1

Abstract

Background: The chitinase-like protein, YKL-40, plays an important role in allergic diseases pathogenesis. Allergens induce its production from macrophages and epithelial cells. Some investigations indicated that Loratadine has anti-inflammatory effects apart from its H1 receptors antagonist activity. We made an attempt to know whether human epithelial cells and macrophages stimulated for YKL-40 synthesis by house dust mite (HDM) extract might be influenced by Loratadine. We used the human pulmonary type II epithelial cell line A549 and the human monocytic cell line (THP-1) for our in vitro study. Materials and method: In the present experimental study, after dose-finding studies using neutral red cytotoxicity assay, the cells were pre-incubated with Loratadine (0. 5 μ g/ml) and then stimulated using HDM extract (GREER Lab). Also, in post-treatment condition, A549 and THP1-cells were stimulated with HDM extract followed by incubation with Loratadine. Finally, the amount of YKL-40 released into the cell supernatants was determined using a specific ELISA. Data were analyzed using SPSS software, v. 16. Results: A549 cells cannot produce and secrete YKL-40 at baseline level or in HDM stimulated condition. In spite of A549, THP-1 cells stimulated with HDM extract showed a significantly increased release of YKL-40. In comparison with control cells, Pre-incubation with Loratadine diminished the YKL-40 release from the HDM stimulated THP1-cells in a significant manner. The YKL40-level in Loratadine group was 4470 pg/ml while it was 8700 pg/ml in the control group (P≤ 0. 05). Although in HDM pretreated cells followed by incubation with Loratadine YKL-40, no decrease was found. The Neutral Red Assay results represented nontoxicity of treatments. Conclusions: A549 cells that are used widely in in-vitro models of allergic reactions cannot produce YKL-40 as a mediator of allergic reactions. Therefore, this cell line should not be used alone in these studies. Loratadine may have anti-inflammatory effects by suppressing YKL-40 secretion from myeloid cells. As for the ability of Loratadine to attenuate YKL-40, we suggest that further studies are warranted to examine its therapeutic potential in diseases that YKL-40 plays a critical role in the pathogenesis.

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APA: Copy

Baqernezhadan, Zeinab, Darbandi Tamijani, Hasan, & Hajimollahoseini, Mostafa. (2019). Effect of loratadine on YKL-40 production in Mite extract stimulated A549 and THP-1 cells. PAJOUHESH DAR PEZESHKI, 42(4 ), 202-208. SID. https://sid.ir/paper/373248/en

Vancouver: Copy

Baqernezhadan Zeinab, Darbandi Tamijani Hasan, Hajimollahoseini Mostafa. Effect of loratadine on YKL-40 production in Mite extract stimulated A549 and THP-1 cells. PAJOUHESH DAR PEZESHKI[Internet]. 2019;42(4 ):202-208. Available from: https://sid.ir/paper/373248/en

IEEE: Copy

Zeinab Baqernezhadan, Hasan Darbandi Tamijani, and Mostafa Hajimollahoseini, “Effect of loratadine on YKL-40 production in Mite extract stimulated A549 and THP-1 cells,” PAJOUHESH DAR PEZESHKI, vol. 42, no. 4 , pp. 202–208, 2019, [Online]. Available: https://sid.ir/paper/373248/en

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