Molecular Analysis and Typing of Methicillin Resistance Staphylococcus Aureus Strains Isolated from Hospitalized Patients

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Abiri Parnaz; AKHAVAN SEPAHI ABBAS; GOUDARZI MEHDI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Research in Medicine Year:2018 | Volume:42 | Issue:3 Page(s): 167-175

Download Full-Text

Persian Verion

View:

631

Download:

Cites:

Information Journal Paper

Title

Molecular Analysis and Typing of Methicillin Resistance Staphylococcus Aureus Strains Isolated from Hospitalized Patients

Author(s)

Abiri Parnaz | AKHAVAN SEPAHI ABBAS | GOUDARZI MEHDI | Issue Writer Certificate

Keywords

S.aureusQ2

SCCmecQ1

MRSAQ1

Abstract

Background: The prevalence of StaphylococcuS.aureus, as one of the most common nosocomial pathogens, is increasing annually and is becoming a major public health concern. One of the serious threats associated with clinical isolates of MRSA is lack of data regarding the molecular characterization of these isolates. The aim of the present study was to identify resistance encoding genes and molecular characteristics of methicillin-resistant S. aureus strains isolated from hospitalized patients in 2017. Materials and Methods: During a 10-month period, 112 MRSA strains isolated from hospitalized patients were investigated. In vitro antimicrobial susceptibility of the isolates was assessed using the disk diffusion and micro-broth dilution methods. Conventional PCR was performed to detect resistance encoding genes. Different types of SCCmec were analyzed using multiplex PCR. Results: The results of antibiotic susceptibility testing showed that 91. 1% of isolates were resistant to penicillin, 65. 2% to ceftriaxon, 63. 4% to erythromycin, 56. 3% to kanamycin, 52. 7% to Clindamycin, 50% to amikacin, 45. 5% to gentamicin, 26. 8% to tobramycin, 18. 8% to quinupristin/dalfopristin, and 11. 6% were resistant to trimetoprim-sulfamethoxazole. The most prevalent resistance gene belonged to ant(4΄ )Ia (73. 2%) followed by aac(6΄ )-Ie/aph(2˝ ) (59. 8%), tet(M) (57. 1%), msr(A) (36. 7%), aph(3΄ )-IIIa (35. 7%), erm(A) (33. 9%), msr(B) (24. 1%), erm(B) (17%), erm(C) (15. 2%), and mupA (10. 7%). Our findings revealed that the most common SCCmec type was III (53. 6%) followed by types I (23. 3%), IV (14. 3%), and II (8. 9%). High-level mupirocin-resistant strains belonged to SCCmec types III (5. 4%), IV (4. 4%), and I (0. 9%), while all the low-level mupirocin resistant strains belonged to SCCmec type III (15. 2%). Conclusions: It seems that there is a genetic diversity among MRSA circulating in studied hospitals that highlights the need to implement appropriate infection control policies in order to decrease dissemination of multi-drug resistance MRSA types in our hospitals.

Cites

No record.

References

No record.

Cite

APA: Copy

Abiri, Parnaz, AKHAVAN SEPAHI, ABBAS, & GOUDARZI, MEHDI. (2018). Molecular Analysis and Typing of Methicillin Resistance Staphylococcus Aureus Strains Isolated from Hospitalized Patients. PAJOUHESH DAR PEZESHKI, 42(3 ), 167-175. SID. https://sid.ir/paper/374165/en

Vancouver: Copy

Abiri Parnaz, AKHAVAN SEPAHI ABBAS, GOUDARZI MEHDI. Molecular Analysis and Typing of Methicillin Resistance Staphylococcus Aureus Strains Isolated from Hospitalized Patients. PAJOUHESH DAR PEZESHKI[Internet]. 2018;42(3 ):167-175. Available from: https://sid.ir/paper/374165/en

IEEE: Copy

Parnaz Abiri, ABBAS AKHAVAN SEPAHI, and MEHDI GOUDARZI, “Molecular Analysis and Typing of Methicillin Resistance Staphylococcus Aureus Strains Isolated from Hospitalized Patients,” PAJOUHESH DAR PEZESHKI, vol. 42, no. 3 , pp. 167–175, 2018, [Online]. Available: https://sid.ir/paper/374165/en

Related Journal Papers