Effect of miRNA-1266-5p repression on the increasing cell survival and alterations of the cell cycle in AGS cell line

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Sorayyayi Saba; Vahidi Sogand; MOHAMMADZADEH MOHAMMAD; Hosseini Asl Sayyed Saied

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2018 | Volume:76 | Issue:8 Page(s): 562-567

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Title

Effect of miRNA-1266-5p repression on the increasing cell survival and alterations of the cell cycle in AGS cell line

Author(s)

Sorayyayi Saba | Vahidi Sogand | MOHAMMADZADEH MOHAMMAD | Hosseini Asl Sayyed Saied | Issue Writer Certificate

Keywords

cell cycleQ1

cell survivalQ1

gastric cancerQ2

miRNA-1266Q1

Abstract

Background: gastric cancer is among the most common malignancies in certain parts of the world, such as northwest Iran. miRNAs are small and single-stranded noncoding RNAs with about 19-23 nucleotides. Several studies have shown that miRNAs play important roles in gastric tumorigenesis. The aim of this study was to determine the effect of miRNA-1266-5p repression on the cell survival and alterations of the cell cycle in gastric cancer cell line of AGS (NCBI Code: C131, Gastric epithelial cell line). Methods: This experimental study was performed from April to December 2017 in Cellular-Molecular Research Center of Ardabil University of Medical Sciences, Iran. In this study, AGS cells were cultured in RPMI-1640 medium containing 10% serum and 1% antibiotic. The cells were transfected with miR-1266-5p mimic, miR-1266-5p inhibitor and HiPerFect reagent alone as negative control. The miR-1266-5p expression and transfection efficiency were analyzed by Stem-loop TaqMan qRT-PCR. The cell proliferation and cell cycle alterations were determined using MTT calorimetric assay and flow cytometry, respectively. The results were analyzed using SPSS 19. 0 statistics software (SPSS Inc., Chicago, IL, USA) and presented as the means± standard deviation (SD). Results: miR-1266-5p expression was increased in AGS cells transfected with miR-1266-5p mimic compared to control cells (P=0), while miR-1266-5p expression was decreased in transfected cells with the inhibitor compared to controls (P=0). Among different time points, the most effects of miR-1266-5p mimic and inhibitor were noticed after 48 hours of transfection. The upregulated miR-1266-5p significantly decreased cell growth, in contrast, inhibitor promoted cell proliferation (P=0). In addition, miR-1266-5p upregulation induced cell cycle arrest at the transition of G1 to S phase and led to G0/G1 entry (P=0), while of miR-1266-5p led to G2/M entry (P=0. 001). Conclusion: According to the results obtained from this study, miR-1266-5p can reduce cell survival and induce cell cycle arrest and act as a tumor suppressor in AGS cells. While its inhibition can increase cell survival and reduce apoptosis.

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APA: Copy

Sorayyayi, Saba, Vahidi, Sogand, MOHAMMADZADEH, MOHAMMAD, & Hosseini Asl, Sayyed Saied. (2018). Effect of miRNA-1266-5p repression on the increasing cell survival and alterations of the cell cycle in AGS cell line. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 76(8 ), 562-567. SID. https://sid.ir/paper/376654/en

Vancouver: Copy

Sorayyayi Saba, Vahidi Sogand, MOHAMMADZADEH MOHAMMAD, Hosseini Asl Sayyed Saied. Effect of miRNA-1266-5p repression on the increasing cell survival and alterations of the cell cycle in AGS cell line. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2018;76(8 ):562-567. Available from: https://sid.ir/paper/376654/en

IEEE: Copy

Saba Sorayyayi, Sogand Vahidi, MOHAMMAD MOHAMMADZADEH, and Sayyed Saied Hosseini Asl, “Effect of miRNA-1266-5p repression on the increasing cell survival and alterations of the cell cycle in AGS cell line,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 76, no. 8 , pp. 562–567, 2018, [Online]. Available: https://sid.ir/paper/376654/en

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