THE EFFECT OF 8 DAY STORAGE AT 4˚C ON TOTAL NUCLEATED CELL COUNT, CELL VIABILITY, AND GRANULOCYTE-MACROPHAGE COLONY FORMING UNIT OF MOBILIZED PERIPHERAL BLOOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHY ALDIN BONAB M.; ALIMOGHADDAM K.A.; POOYA A.P.; BESHTAR M.; GHAVAMZADEH A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Tehran University Medical Journal Year:2004 | Volume:62 | Issue:1 Page(s): 1-15

Download Full-Text

Persian Verion

View:

769

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF 8 DAY STORAGE AT 4˚C ON TOTAL NUCLEATED CELL COUNT, CELL VIABILITY, AND GRANULOCYTE-MACROPHAGE COLONY FORMING UNIT OF MOBILIZED PERIPHERAL BLOOD

Author(s)

Keywords

PERIPHERAL BLOOD PROGENITOR CELLQ4

REFRIGERATIONQ4

GM-CFUQ4

Abstract

Background: Hematopoietic stem cell (HSC) transplantation has brought the possibility of the use of high dose chemotherapy in the treatment of malignant hematopoietic diseases. Short-term HSC preservation at 4˚C is the most common method for autologous peripheral blood stem cell transplantation (PBSCT). Materials and Methods: Thirty-seven mobilized PBSC samples from thirteen hematological patients (4 AML, 4 MM and 5 Lymphoma cases) who were selected for autologous PBSCT and 24 normal candidates for allogenic PBSCT were preserved in five separate sterile 2 ml tubes in 4˚C. Each sample was evaluated for total nucleated cell (TNC) count, dye exclusion cell viability and Granulocyte-Macrophage colony forming unit (GM-CFU; in semisolid medium after 16 days) in days 0, 2, 4, 6 and 8. The results were converted to percentages of day 0 measures. The data were analyzed by SPSS 10.0 using Paired Samples T test, Independent Samples T test and Regression.Results: The mean percentages (and standard deviations) of TNC count, cell viability and GM-CFU for days 0, 2, 4, 6 and 8 are shown below: No significant correlation was found between age, sex, weight and the kind of donor with TNC, viability and GM-CFU.Conclusion: In this study, we have found that during storage of mobilized PBSC in 4˚C, TNC count and cell viability still remains higher than 70% after eight days, while GM-CFU decreases more rapidly and falls to less than 50% after day 4.Therefore, TNC count and cell viability do not decrease as fast as GM-CFU.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHY ALDIN BONAB, M., ALIMOGHADDAM, K.A., POOYA, A.P., BESHTAR, M., & GHAVAMZADEH, A.. (2004). THE EFFECT OF 8 DAY STORAGE AT 4˚C ON TOTAL NUCLEATED CELL COUNT, CELL VIABILITY, AND GRANULOCYTE-MACROPHAGE COLONY FORMING UNIT OF MOBILIZED PERIPHERAL BLOOD. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 62(1), 1-15. SID. https://sid.ir/paper/38017/en

Vancouver: Copy

MOHY ALDIN BONAB M., ALIMOGHADDAM K.A., POOYA A.P., BESHTAR M., GHAVAMZADEH A.. THE EFFECT OF 8 DAY STORAGE AT 4˚C ON TOTAL NUCLEATED CELL COUNT, CELL VIABILITY, AND GRANULOCYTE-MACROPHAGE COLONY FORMING UNIT OF MOBILIZED PERIPHERAL BLOOD. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2004;62(1):1-15. Available from: https://sid.ir/paper/38017/en

IEEE: Copy

M. MOHY ALDIN BONAB, K.A. ALIMOGHADDAM, A.P. POOYA, M. BESHTAR, and A. GHAVAMZADEH, “THE EFFECT OF 8 DAY STORAGE AT 4˚C ON TOTAL NUCLEATED CELL COUNT, CELL VIABILITY, AND GRANULOCYTE-MACROPHAGE COLONY FORMING UNIT OF MOBILIZED PERIPHERAL BLOOD,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 62, no. 1, pp. 1–15, 2004, [Online]. Available: https://sid.ir/paper/38017/en

Related Journal Papers