THE EFFECT OF DIFFERENT DOSES OF HYALURONAN ON SPERM MORPHOLOGY, MOTILITY, VITALITY AND FERTILIZATION CAPABILITY IN MOUSE

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SAYADI S.; AKBARI M.; SOBHANI A.; ABOLHASANI F.; TAKZARE N.; PASBAKHSH P.

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Journal: Tehran University Medical Journal Year:2006 | Volume:64 | Issue:6 Page(s): 27-36

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Title

THE EFFECT OF DIFFERENT DOSES OF HYALURONAN ON SPERM MORPHOLOGY, MOTILITY, VITALITY AND FERTILIZATION CAPABILITY IN MOUSE

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Keywords

HYALURONANQ3

SPERM MOTILITYQ3

SPERM MORPHOLOGYQ3

IVFQ4

Abstract

Background: HYALURONAN has an important role on the permeability and motility of sperm and the interaction of gametes and these can play a considerable role on the fertility rate. Therefore, in this study, we assessed the effect of different doses of HYALURONAN on the morphology, motility, vitality and fertility rate of mice.Methods: We used 40 mice (6-8 week) in this study which twenty of them were male and the rest were female. The sperm of each male mouse were divided into four groups.The group 1 (control): They were maintained in RPMI media without any HYALURONAN supplementation for 2 hour. HYALURONAN with the doses of 750, 1000 and 1250 mg/ml were added into RPMI media in groups 2, 3 and 4, respectively. After 2 hour.incubation, the numbers of sperms were assessed, using haemocytometer. Also, their morphology with papanicolaeu staining and their vitality with Eosin B dye were assessed. As well as sperms motility measured under inverted microscope by observation and fertility rate evaluated after routine IVF by counting two-cell stage embryos.Results: Our results demonstrated that, the dose of 750 mg/ml has the greatest effect on the motility, vitality and fertility rate of sperms. The effect of dose of 1000 M g/ml also was positive on them. On the other hand, none of these doses had any effect on SPERM MORPHOLOGY.Conclusion: HYALURONAN may have an influence on motility, vitality and fertility rate of sperms and the dose of 750m g/ml had a significant effect on these factors.

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APA: Copy

SAYADI, S., AKBARI, M., SOBHANI, A., ABOLHASANI, F., TAKZARE, N., & PASBAKHSH, P.. (2006). THE EFFECT OF DIFFERENT DOSES OF HYALURONAN ON SPERM MORPHOLOGY, MOTILITY, VITALITY AND FERTILIZATION CAPABILITY IN MOUSE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 64(6), 27-36. SID. https://sid.ir/paper/38024/en

Vancouver: Copy

SAYADI S., AKBARI M., SOBHANI A., ABOLHASANI F., TAKZARE N., PASBAKHSH P.. THE EFFECT OF DIFFERENT DOSES OF HYALURONAN ON SPERM MORPHOLOGY, MOTILITY, VITALITY AND FERTILIZATION CAPABILITY IN MOUSE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2006;64(6):27-36. Available from: https://sid.ir/paper/38024/en

IEEE: Copy

S. SAYADI, M. AKBARI, A. SOBHANI, F. ABOLHASANI, N. TAKZARE, and P. PASBAKHSH, “THE EFFECT OF DIFFERENT DOSES OF HYALURONAN ON SPERM MORPHOLOGY, MOTILITY, VITALITY AND FERTILIZATION CAPABILITY IN MOUSE,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 64, no. 6, pp. 27–36, 2006, [Online]. Available: https://sid.ir/paper/38024/en

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