Evaluation of Genomic Polymorphism of Candida Albicans, Isolated from the Patients with Vaginitis by RAPD-PCR Method

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Mofidian f.; AMINI K.; Kazemy m.j.

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Journal: JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:27 | Issue:108 Page(s): 1-10

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Title

Evaluation of Genomic Polymorphism of Candida Albicans, Isolated from the Patients with Vaginitis by RAPD-PCR Method

Author(s)

Mofidian f. | AMINI K. | Kazemy m.j. | Issue Writer Certificate

Keywords

Candida AlbicansQ1

polymorphismQ1

Random Amplified Polymorphic DNA TechniqueQ3

VaginitisQ3

Abstract

Introduction: Candida Albicans are human selective symbiosis, mainly located in the gastrointestinal tract. This group of fungi is pathogenic where host resistance reduces to local or systemic infection. Bacteria are the most common cause of Vaginitis, but Candida Albicans are the second leading cause of such infections. Candida Albicans are responsible for 70-90% of the fungal infections in the vaginal area. objective: To study the genomic polymorphism of Candida Albicans isolated from women with Vaginitis using RAPDPCR method. Materials and Methods: This cross-sectional study was designed and implemented by descriptive-analytic method. In this research, 60 samples of 30 vaginal samples from Shariati Hospital (Tehran) and 30 samples from Kerman Women's Hospital were collected by gynecologist and obstetrician. Using a sterile loop, various species yeasts were cultured on a differential chromatographed medium of Candida agar. Differentiation of subspecies and study of the possible relationship between specific strains and clinical forms was done. Typing of Albicans isolates was also done using RAPD-PCR method with random primers. Results: Increased non-albicans and resistance to drugs not only in different parts of Iran but also in the world have been reported. In this study, based on the results of culture and direct experiment, 60 people were found to be infected with candida volvaginitis infection in two provinces of Kerman and Tehran, which were genetically similar and different in 14 clusters. Some clusters have repeated strains indicating similarity to genomic strains. Conclusion: There are several methods for genomic fingerprinting of different types of clinical isolates of Candida Albicans, using Pulsed Field Gel Electrophoresis (Pulsed Field Gel Electrophoresis) technique, fingerprinting methods based on ribotaiping, and fingerprinting methods based on PCR for epidemiological outbreaks are useful.

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APA: Copy

Mofidian, f., AMINI, K., & Kazemy, m.j.. (2019). Evaluation of Genomic Polymorphism of Candida Albicans, Isolated from the Patients with Vaginitis by RAPD-PCR Method. JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, 27(108 ), 1-10. SID. https://sid.ir/paper/384792/en

Vancouver: Copy

Mofidian f., AMINI K., Kazemy m.j.. Evaluation of Genomic Polymorphism of Candida Albicans, Isolated from the Patients with Vaginitis by RAPD-PCR Method. JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;27(108 ):1-10. Available from: https://sid.ir/paper/384792/en

IEEE: Copy

f. Mofidian, K. AMINI, and m.j. Kazemy, “Evaluation of Genomic Polymorphism of Candida Albicans, Isolated from the Patients with Vaginitis by RAPD-PCR Method,” JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 108 , pp. 1–10, 2019, [Online]. Available: https://sid.ir/paper/384792/en

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