SURVEY RATE ISOLATION OF HEAMOPHILUS INFLUENZAE TYPE B IN CSF OF PEDIATRICS SUSPECTED TO MENINGITIS BY CULTURE AND PCR IN TEHRAN CENTER OF MEDICAL CHILDREN

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TAHERI KALANI M.; AKBARI NAKHJAVANI F.; KAZEMI BAHRAM; BONAKDAR HASHEMI F.; HAGHI ASHTEIANI M.; NOURI KERAMAT; ERFANI Y.; ABEDINI MINA; SHAHSAVAN SH.; PEYMANI A.

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Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2006 | Volume:63 | Issue:12 Page(s): 1006-1014

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Title

SURVEY RATE ISOLATION OF HEAMOPHILUS INFLUENZAE TYPE B IN CSF OF PEDIATRICS SUSPECTED TO MENINGITIS BY CULTURE AND PCR IN TEHRAN CENTER OF MEDICAL CHILDREN

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Keywords

HEAMOPHILUS INFLUENZAE TYPE BQ3

CSFQ4

MENINGITISQ3

CULTURE AND PCRQ3

Abstract

Background: Bacterial MENINGITIS is a serious and occationaly lethal infection that affects central nerves system. Reamophilus influenzae type b, streptococcus pneumonia, E. coli, and streptococcus agalactiae are agent 95% of pediatrics MENINGITIS.Materials and Methods: Custom laboratory methods such as culture that used for detection agent-caused MENINGITIS take up 36 hours. In addition following antimicrobial therapy prior sapling, its found that efficacy of culture in detection of MENINGITIS caused microorganisms is about 30 %. Then non-culture methods such PCR are used. Thirty thousand of CSF from Tehran Medical Center of Children was collected during from 1381 to 1382. Then CULTURE AND PCR by using specific primers was done for detection.Results: Thirty-two samples out of 300 total samples was culture positive, that Rib was detected in 5 cases. In addition of five cultural positive cases of Rib, two other cases were detected by PCR.Conclusion: This study showed that Rib is agent 15.6% of pediatrics bacterial MENINGITIS. Also comparison CULTURE AND PCR showed that later method have sensitivity and specificity, 100% and 99%, respectively. With regarding to rapid detection and cure of MENINGITIS is essential, then providing exiting instrument for PCR, using of this method is advised.

Cites

ETIOLOGY OF ACUTE BACTERIAL MENINGITIS IN IRAN: A SYSTEMATIC REVIEW

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APA: Copy

TAHERI KALANI, M., AKBARI NAKHJAVANI, F., KAZEMI, BAHRAM, BONAKDAR HASHEMI, F., HAGHI ASHTEIANI, M., NOURI, KERAMAT, ERFANI, Y., ABEDINI, MINA, SHAHSAVAN, SH., & PEYMANI, A.. (2006). SURVEY RATE ISOLATION OF HEAMOPHILUS INFLUENZAE TYPE B IN CSF OF PEDIATRICS SUSPECTED TO MENINGITIS BY CULTURE AND PCR IN TEHRAN CENTER OF MEDICAL CHILDREN. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 63(12), 1006-1014. SID. https://sid.ir/paper/38721/en

Vancouver: Copy

TAHERI KALANI M., AKBARI NAKHJAVANI F., KAZEMI BAHRAM, BONAKDAR HASHEMI F., HAGHI ASHTEIANI M., NOURI KERAMAT, ERFANI Y., ABEDINI MINA, SHAHSAVAN SH., PEYMANI A.. SURVEY RATE ISOLATION OF HEAMOPHILUS INFLUENZAE TYPE B IN CSF OF PEDIATRICS SUSPECTED TO MENINGITIS BY CULTURE AND PCR IN TEHRAN CENTER OF MEDICAL CHILDREN. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2006;63(12):1006-1014. Available from: https://sid.ir/paper/38721/en

IEEE: Copy

M. TAHERI KALANI, F. AKBARI NAKHJAVANI, BAHRAM KAZEMI, F. BONAKDAR HASHEMI, M. HAGHI ASHTEIANI, KERAMAT NOURI, Y. ERFANI, MINA ABEDINI, SH. SHAHSAVAN, and A. PEYMANI, “SURVEY RATE ISOLATION OF HEAMOPHILUS INFLUENZAE TYPE B IN CSF OF PEDIATRICS SUSPECTED TO MENINGITIS BY CULTURE AND PCR IN TEHRAN CENTER OF MEDICAL CHILDREN,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 63, no. 12, pp. 1006–1014, 2006, [Online]. Available: https://sid.ir/paper/38721/en

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