THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE

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PIRMOHAMMAD JAMAAT ZEYNAB; HOSSEINI EHTERAMOLSADAT; GHASEMZADEH MEHRAN

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2016 | Volume:74 | Issue:2 Page(s): 92-98

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Title

THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE

Author(s)

PIRMOHAMMAD JAMAAT ZEYNAB | HOSSEINI EHTERAMOLSADAT | GHASEMZADEH MEHRAN | Issue Writer Certificate

Keywords

GLYCOCALICINQ1

GPIBαQ1

PLATELET ACTIVATIONQ1

PLATELET-RICH PLASMAQ2

THROMBOSISQ1

Abstract

Background: Platelet adhesion typically occurs by the critical role of GPIb-V-IX in capturing free-flowing platelets to the injured vessel wall where its rapid binding kinetics enables platelet tethering even under conditions of high shear through the interaction of the major ligand-binding subunit of GPIb-V-IX, GPIbα with subendothelial-bound vWF. During storage, platelet undesired activation may lead to platelet storage lesion (PSL) which changes the expression levels of platelet functional receptors including GPIbα. This study investigates the levels of expression and ectodomain shedding of platelet adhesive receptor GPIba during the storage of platelet rich plasma (PRP) concentrates (PRP- PCs).Methods: Five PRP-platelet concentrates were obtained from Iranian Blood Transfusion Organization (IBTO). The GPIba expressions of platelets were analyzed on day 1, 3 and 5 after storage using flowcytometry. To examine the ectodomain shedding of this receptor the microparticle free supernatants obtained from stored platelets were subjected to western blot analysis. For control study, blood specimens was drawn from healthy consenting individuals and resting platelets were isolated while resuspended in Tyrode buffer.Results: Our results indicated a continuous decrease of GPIba expression during storage where the expression from fist day (Mean fluorescence intensity=86±5.9) was significantly reduced compared to that of fifth day (mean fluorescence intensity=61±7.7) after storage (P=0.0094). Conversely, shed GPIba (GLYCOCALICIN) demonstrated continuous elevation during five-day storage (P=0.0098). According to the results the shedding levels for the first day were increased from 0.31±0.3 to 1.5±0.4 by the day 5 after storage.Conclusion: Our study has demonstrated significant loss of platelet GPIba during storage mostly due to receptor ectodomain shedding that leads to significant increase of soluble GPIba in stored platelets. Considering the high levels of shed GPIba in long stored platelets whether the transfusion of such products might be associated with defective adhesive function of platelets or possible proinflammatory effects could be of interests for future investigation.

Cites

LEUKOCYTES AND TRANSFUSION RELATED ADVERSE EVENTS: THE EFFECTS OF LEUKOREDUCTION PROCESS IN THE PREVENTION OF ADVERSE REACTIONS RESULTED FROM THE TRANSFUSION OF BLOOD COMPONENTS: REVIEW ARTICLE

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APA: Copy

PIRMOHAMMAD JAMAAT, ZEYNAB, HOSSEINI, EHTERAMOLSADAT, & GHASEMZADEH, MEHRAN. (2016). THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 74(2), 92-98. SID. https://sid.ir/paper/38916/en

Vancouver: Copy

PIRMOHAMMAD JAMAAT ZEYNAB, HOSSEINI EHTERAMOLSADAT, GHASEMZADEH MEHRAN. THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2016;74(2):92-98. Available from: https://sid.ir/paper/38916/en

IEEE: Copy

ZEYNAB PIRMOHAMMAD JAMAAT, EHTERAMOLSADAT HOSSEINI, and MEHRAN GHASEMZADEH, “THE EXPRESSION LOSS OF GPIBα DUE TO ECTODOMAIN SHEDDING IN PRP DERIVED PLATELET CONCENTRATES DURING STORAGE,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 74, no. 2, pp. 92–98, 2016, [Online]. Available: https://sid.ir/paper/38916/en

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