Neuroprotective effect of topiramate against 6-hydroxydopamine-induced cell death in Parkinson's disease cell model

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Fallah Fahimeh; Aminzadeh Azadeh

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Journal Paper

Paper Information

Journal: Koomesh Year:2019 | Volume:21 | Issue:4 (76) Page(s): 702-707

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Information Journal Paper

Title

Neuroprotective effect of topiramate against 6-hydroxydopamine-induced cell death in Parkinson's disease cell model

Author(s)

Fallah Fahimeh | Aminzadeh Azadeh | Issue Writer Certificate

Keywords

6-HydroxydopamineQ2

Oxidative StressQ2

Parkinson DiseaseQ2

TopiramateQ2

PC12 cellsQ2

Abstract

Introduction: Parkinson's disease (PD) is a common neurodegenerative disorder characterized by progressive neuronal dysfunction. Growing evidence has shown that Oxidative Stress plays a crucial role in the pathogenesis of Parkinson's disease. Correspondingly, the current study evaluated the protective effect of Topiramate in 6hydroxydopamine induced Oxidative Stress and apoptosis in PC12 cells as an in vitro model for PD. Materials and Methods: PC12 cells, a cellular model of PD, were treated with Topiramate for 24 h. Then they were treated with 75 μ M 6-Hydroxydopamine for 24 h. Cell viability was examined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5diphenyl-2-tetrazolium bromide (MTT) assay. Intracellular reactive oxygen species (ROS) levels were measured using 2, 7 dichlorodihydrofluorescein diacetate (DCF-DA) assay. The levels of lipid peroxidation and total antioxidant power (TAP) were also measured. Results: Remarkably, Topiramate at concentration of 8 μ M increased survival of PC12 cells exposed to 6hydroxydopamine. Cell viability of PC12 cells on 6-Hydroxydopamine was decreased compared to controls, which was reversed by Topiramate. Topiramate also reduced intracellular reactive oxygen species (ROS) level and cellular lipid peroxidation. In addition, Topiramate significantly increased total antioxidant power in 6-OHDA-treated cells. Conclusion: Findings of this study showed that the Topiramate protects PC12 cells against 6-OHDA-induced neurotoxicity through its potent antioxidant activity.

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APA: Copy

Fallah, Fahimeh, & Aminzadeh, Azadeh. (2019). Neuroprotective effect of topiramate against 6-hydroxydopamine-induced cell death in Parkinson's disease cell model. KOOMESH, 21(4 (76) ), 702-707. SID. https://sid.ir/paper/389187/en

Vancouver: Copy

Fallah Fahimeh, Aminzadeh Azadeh. Neuroprotective effect of topiramate against 6-hydroxydopamine-induced cell death in Parkinson's disease cell model. KOOMESH[Internet]. 2019;21(4 (76) ):702-707. Available from: https://sid.ir/paper/389187/en

IEEE: Copy

Fahimeh Fallah, and Azadeh Aminzadeh, “Neuroprotective effect of topiramate against 6-hydroxydopamine-induced cell death in Parkinson's disease cell model,” KOOMESH, vol. 21, no. 4 (76) , pp. 702–707, 2019, [Online]. Available: https://sid.ir/paper/389187/en

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