Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Nouri Hoda; MOGHIMI HAMID; KHALEGHIAN ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Koomesh Year:2020 | Volume:22 | Issue:1 (77) Page(s): 178-184

Download Full-Text

Persian Verion

View:

272

Download:

Cites:

Information Journal Paper

Title

Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran

Author(s)

Nouri Hoda | MOGHIMI HAMID | KHALEGHIAN ALI | Issue Writer Certificate

Keywords

AsparaginaseQ1

YeastQ2

LeukemiaQ2

IranQ2

Abstract

Introduction: The use of L-Asparaginase as a chemotherapeutic agent is an effective strategy for the treatment of leukemic lymphoblasts. Since the use of bacterial L-Asparaginase causes side effects due to hypersensitivity reactions, search for new enzyme sources is one of the active research areas. In this study, the characterization of produced LAsparaginase by indigenous Yeast strains was investigated. Material and methods: In this study, 130 Yeast strains were evaluated. Remarkably, AG90 isolate with 0. 94 IU/ml enzyme production was selected as the superior strain. Results: The molecular identification of the AG90 showed that this strain exhibited a high level of similarity (99%) with Sarocladium sp. and introduced as a Sarocladium sp. AG90. The fermentation broth of the superior strain showed that 86% of the enzyme was produced extracellularly. The Km and Vmax values of the produced enzyme for the LAsparagine substrate were calculated as 9. 74 mM and 19. 19 μ mol/min, respectively. Discussion: The obtained results in this study can help to introduce a new enzyme with eukaryotic origin to reduce the toxicity, sensitivity and side effects associated of drug consumption.

Cites

No record.

References

No record.

Cite

APA: Copy

Nouri, Hoda, MOGHIMI, HAMID, & KHALEGHIAN, ALI. (2020). Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran. KOOMESH, 22(1 (77) ), 178-184. SID. https://sid.ir/paper/389214/en

Vancouver: Copy

Nouri Hoda, MOGHIMI HAMID, KHALEGHIAN ALI. Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran. KOOMESH[Internet]. 2020;22(1 (77) ):178-184. Available from: https://sid.ir/paper/389214/en

IEEE: Copy

Hoda Nouri, HAMID MOGHIMI, and ALI KHALEGHIAN, “Production and characterization of biochemical properties of L-Asparaginase by indigenous yeast isolated from soil of Iran,” KOOMESH, vol. 22, no. 1 (77) , pp. 178–184, 2020, [Online]. Available: https://sid.ir/paper/389214/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops