IDENTIFICATION OF CANDIDA SPECIES ASSOCIATED WITH VULVOVAGINAL CANDIDIASIS BY COMPARISON OF AN INNOVATIVE MOLECULAR METHOD WITH CULTURE IN 2013

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SOHRABI H.; SAROOKHANI M.R.; EZANI A.

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Journal Paper

Paper Information

Journal: Journal of Arak University Medical Sciences Year:2013 | Volume:16 | Issue:8 (77) Page(s): 0-0

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Title

IDENTIFICATION OF CANDIDA SPECIES ASSOCIATED WITH VULVOVAGINAL CANDIDIASIS BY COMPARISON OF AN INNOVATIVE MOLECULAR METHOD WITH CULTURE IN 2013

Author(s)

SOHRABI H. | SAROOKHANI M.R. | EZANI A. | Issue Writer Certificate

Keywords

CANDIDAQ2

CULTUREQ2

POLYMERASE CHAIN REACTIONQ2

VULVOVAGINITISQ2

Abstract

Background: Vulvovaginal candidiasis is a common problem in women. The purpose of this study was to compare two identification methods; new PCR analysis (with DNA extracted from samples) and conventional CULTURE technique in detection of CANDIDA species in vulvovaginal samples.Material and Methods: In this experimental-analytical study, 150 women of fertility ages participated and two vaginal discharge samples were collected by speculum. One sample used for direct DNA extraction as well as PCR and the other was used for CULTURE and phenotypic evaluations.Phenotypic evaluations waere performed by germ tube and chlamydospore formation and specially CULTURE in chrome agar medium to detect color of the colonies. PCR was performed by DNA extracted from samples as templates and finally size of CANDIDA specific amplicons was determined.Results: From 150 samples, 87 in CULTURE and 127 in new PCR technique were positive. In CULTURE method, from total 87 CANDIDA species, 73 C. albicans, 12 C. glabrata and 2 C. tropicalis were isolated whereas in new PCR technique, from total 127 CANDIDA species 107 C. albicans, 18 C. glabrata and 2 C. tropicalis were identified. Concordance of the two methods were calculated as 68 percent.Conclusions: The new molecular method (innovative PCR) has the potential to rapidly and accurately diagnose CANDIDA VULVOVAGINITIS in patients and can be used for diagnosis of CANDIDA species in clinical specimens.

Cites

MOLECULAR IDENTIFICATION OF CANDIDA SPECIES ISOLATED FROM PATIENTS WITH VULVOVAGINITIS FOR THE FIRST TIME IN MASHHAD

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APA: Copy

SOHRABI, H., SAROOKHANI, M.R., & EZANI, A.. (2013). IDENTIFICATION OF CANDIDA SPECIES ASSOCIATED WITH VULVOVAGINAL CANDIDIASIS BY COMPARISON OF AN INNOVATIVE MOLECULAR METHOD WITH CULTURE IN 2013. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 16(8 (77)), 0-0. SID. https://sid.ir/paper/392910/en

Vancouver: Copy

SOHRABI H., SAROOKHANI M.R., EZANI A.. IDENTIFICATION OF CANDIDA SPECIES ASSOCIATED WITH VULVOVAGINAL CANDIDIASIS BY COMPARISON OF AN INNOVATIVE MOLECULAR METHOD WITH CULTURE IN 2013. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2013;16(8 (77)):0-0. Available from: https://sid.ir/paper/392910/en

IEEE: Copy

H. SOHRABI, M.R. SAROOKHANI, and A. EZANI, “IDENTIFICATION OF CANDIDA SPECIES ASSOCIATED WITH VULVOVAGINAL CANDIDIASIS BY COMPARISON OF AN INNOVATIVE MOLECULAR METHOD WITH CULTURE IN 2013,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 16, no. 8 (77), pp. 0–0, 2013, [Online]. Available: https://sid.ir/paper/392910/en

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