DETECTION OF INTRACYTOPLASMIC TH1/TH2 CYTOKINE PROFILES IN PATIENTS WITH SEPSIS AND SEVERE SEPSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHMADINEZHAD Z.; MOBAIEN A.R.; KARIMINIA AMINA; AFHAMI SH.; HATMI Z.N.; TORKABADI E.; YALDA A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Tehran University Medical Journal Year:2007 | Volume:65 | Issue:2 Page(s): 12-17

Download Full-Text

Persian Verion

View:

1,472

Download:

Cites:

Information Journal Paper

Title

DETECTION OF INTRACYTOPLASMIC TH1/TH2 CYTOKINE PROFILES IN PATIENTS WITH SEPSIS AND SEVERE SEPSIS

Author(s)

Keywords

SEPSISQ3

SEVERE SEPSISQ3

CYTOKINEQ3

TH1Q3

TH2Q3

Abstract

Background: SEPSIS is the leading cause of death in critically ill patients throughout the world. The incidence is increasing despite the major advances in the development of antimicrobial agents and other supportive treatments. Based on multiple studies, it has been shown that patient outcome depends on Thl and TH2 CYTOKINE response. Moreover, whenever the TH2 response is predominant, the SEPSIS is more severe. The aim of this study was to evaluate the correlation between CYTOKINE levels and the severity of SEPSIS in patients.Methods: A cross-sectional study on the cellular levels of several proinflammatory CYTOKINEs was carried out in patients with SEPSIS and severe SEPSIS. The study included 37 patients (24 men and 13 women), 26 of them had SEPSIS and 11 had the severe form of SEPSIS Thirty-seven healthy volunteers served as controls. The average age of the patients was 57 years (±23.3 years), with a range of 21 to 92 years. From the whole blood of the subjects, we separated the monocytes and leukocytes, which were then cultured. Using an ELISA method, we measured levels of IFN-y and IL-12 (associated with TH1), and IL-4 and IL-10 (associated with TH2) in the cultured cells with and without cell stimulation. Results: No correlation was found for IFN-g production in the cells of patients with SEPSIS and severe SEPSIS, regardless of whether the patients had died or survived. However, IL-12 levels were significantly decreased in severe SEPSIS compared with those of SEPSIS patients (P=0.048).Furthermore, the cells of expired patients also had significantly decreased IL-12 levels compared with those of surviving patients (P=0.028). We also found that the levels of IFN-g, IL-4, and IL-10 were decreased in patients compared with those of controls, which correlated to their production.However, there was no correlation for IL-12 production between the cells of the patients compared with those of the controls. There was also no correlation for CYTOKINE production between men and women with SEPSIS and in adults compared with that of elderly patients (>55 years old).Conclusion: We have shown that the predominating T helper cell subset in patients with severe SEPSIS, as well as expired patients, is TH2. In conclusion, the correlation of TH1 CYTOKINE production and progression of SEPSIS was demonstrated. Most probably IL-12 levels would be significantly lower in patients with severe SEPSIS and those who expired.

Cites

No record.

References

No record.

Cite

APA: Copy

AHMADINEZHAD, Z., MOBAIEN, A.R., AFHAMI, SH., YALDA, A., KARIMINIA, AMINA, HATMI, Z.N., & TORKABADI, E.. (2007). DETECTION OF INTRACYTOPLASMIC TH1/TH2 CYTOKINE PROFILES IN PATIENTS WITH SEPSIS AND SEVERE SEPSIS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 65(2), 12-17. SID. https://sid.ir/paper/39336/en

Vancouver: Copy

AHMADINEZHAD Z., MOBAIEN A.R., AFHAMI SH., YALDA A., KARIMINIA AMINA, HATMI Z.N., TORKABADI E.. DETECTION OF INTRACYTOPLASMIC TH1/TH2 CYTOKINE PROFILES IN PATIENTS WITH SEPSIS AND SEVERE SEPSIS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2007;65(2):12-17. Available from: https://sid.ir/paper/39336/en

IEEE: Copy

Z. AHMADINEZHAD, A.R. MOBAIEN, SH. AFHAMI, A. YALDA, AMINA KARIMINIA, Z.N. HATMI, and E. TORKABADI, “DETECTION OF INTRACYTOPLASMIC TH1/TH2 CYTOKINE PROFILES IN PATIENTS WITH SEPSIS AND SEVERE SEPSIS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 65, no. 2, pp. 12–17, 2007, [Online]. Available: https://sid.ir/paper/39336/en

Related Journal Papers