STUDY OF THE GENETIC RESEMBLANCE OF TPI GENE IN CAT AND HUMAN GIARDIA USING PCR-RFLP METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

PEZESHKI A.; REZAEIAN M.; ZAREBAVANI M.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2013 | Volume:13 | Issue:2 (48) Page(s): 164-173

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

2,392

Download:

Cites:

Information Journal Paper

Title

STUDY OF THE GENETIC RESEMBLANCE OF TPI GENE IN CAT AND HUMAN GIARDIA USING PCR-RFLP METHOD

Author(s)

PEZESHKI A. | REZAEIAN M. | ZAREBAVANI M. | Issue Writer Certificate

Keywords

PCR-RFLPQ2

GIARDIAQ3

CATQ2

HUMANQ2

Abstract

Background & Objectives: GIARDIA duodenalisis the most common intestinal parasite with cosmopolitan distribution. This parasite has been found in the intestine of HUMANs and other mammalian hosts including CATs, dogs, CATtle, sheep, deer, pigs and muskrats. It is postulated that animals maybe reservoir for HUMAN infection and viceversa. In present study, the possible genetic similarity between CAT and HUMANs GIARDIA and its probable zoonosis were investigated.Methods: Direct examination and formalin-ether concentration techniques were performed on stray and semi stray CAT fecal specimens. Gradient sucrose method was applied for collection and purification of cysts and DNA extraction was performed by phenol-chloroform and CTAB (Cetyl trimethyl ammonium bromid) methods. DNA of cysts could hardly be extracted after repeated freezing and thawing. Polymerase chain reaction (PCR) was performed for DNA amplification. In this study triosephosphate isomerase (tpi) gene was selected as a molecular marker. Two sets of primers (PM 290 and PM 924) were considered. Two restriction enzymes RsaI and AvaI were also used to determine restriction fragment length polymorphism (RFLP) for PCR fragments amplified by both primer sets.Results: Ten samples were positive for GIARDIA cysts which were examined for molecular investigation. Four CAT isolates were amplified by PM 290. PCR-RFLP patterns were found to be similar to HUMAN isolates AC¹AF 069556 (subgroup of AC¹U 57897) with possibility of cross-transmission.Conclusion: Therefore the similarity of genomic characters of isolates of CAT and HUMAN GIARDIA implies possibility of zoonosis and transmission of these protozoa from CAT to HUMAN and vice versa.

Cites

No record.

References

No record.

Cite

APA: Copy

PEZESHKI, A., REZAEIAN, M., & ZAREBAVANI, M.. (2013). STUDY OF THE GENETIC RESEMBLANCE OF TPI GENE IN CAT AND HUMAN GIARDIA USING PCR-RFLP METHOD. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 13(2 (48)), 164-173. SID. https://sid.ir/paper/394676/en

Vancouver: Copy

PEZESHKI A., REZAEIAN M., ZAREBAVANI M.. STUDY OF THE GENETIC RESEMBLANCE OF TPI GENE IN CAT AND HUMAN GIARDIA USING PCR-RFLP METHOD. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2013;13(2 (48)):164-173. Available from: https://sid.ir/paper/394676/en

IEEE: Copy

A. PEZESHKI, M. REZAEIAN, and M. ZAREBAVANI, “STUDY OF THE GENETIC RESEMBLANCE OF TPI GENE IN CAT AND HUMAN GIARDIA USING PCR-RFLP METHOD,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 13, no. 2 (48), pp. 164–173, 2013, [Online]. Available: https://sid.ir/paper/394676/en

Related Journal Papers