Carcinogenic and cytotoxic effects of Diazinon in the mouse: an in vivo and in vitro study

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ASGHARI MOZHGAN; Sadeghi Hashjin Goudarz; Golabchifar Aliakbar; KOOHI MOHAMMAD KAZEM; Mohammadnejad Ahad; RISMANCHI SANAZ; TAHERI MOHAMMAD; SHAMS GHOLAMREZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY Year:2019 | Volume:3 | Issue:3 (11) Page(s): 159-168

Download Full-Text

Persian Verion

View:

225

Download:

Cites:

Information Journal Paper

Title

Carcinogenic and cytotoxic effects of Diazinon in the mouse: an in vivo and in vitro study

Author(s)

Keywords

Organophosphates

Diazinon

Cancer

mice

MTT

Abstract

Background and aims: In this study, the toxicity and carcinogenicity of an organophosphate pesticide, Diazinon, were studied in the mouse by MTT, micronucleus and karyotype methods. Morphological changes of cell lines in the vicinity of Diazinon were also evaluated and recorded. Methods: mice were randomly divided into two groups (n=15 per group): treatment and control groups. In the treatment group, Diazinon (1 ml of 1 ppm concentration) was applied once daily topically on the shaved skin and for 12 weeks; in the controls, physiological salt solution was used. At the end of the period, tissue samples were taken from the organs after autopsy. Murine L929 fibroblast cells were cultured with and without Diazinon (10, 25, 50, 100 μ g/ml). The morphological changes of the cells were evaluated after 48 hours. The cytotoxicity was assessed in vitro by MTT assay and micronucleus and karyotype and IC50 percent were also determined. Results: Microscopic observations of kidney tissue revealed mild cytotoxicity in proximal tubules. Mild hepatotoxicity was also observed in the liver tissue. Morphological results of cell line showed that Diazinon (50 μ g/ml) had a significant effect compared to the control group. The percentage of micronucleus in the group treated with 100 μ g/ml of Diazinon was significantly higher than the control. In the MTT assay, the mean optical absorption at concentrations of 10, 25, 50, 100 μ g/ml was significantly different from that of the control group (P <0. 05). Conclusion: Skin contact with Diazinon induces pathologic effects on organs, cytotoxicity, and procarcinogenic effect in mice.

Cites

No record.

References

No record.

Cite

APA: Copy

ASGHARI, MOZHGAN, Sadeghi Hashjin, Goudarz, Golabchifar, Aliakbar, KOOHI, MOHAMMAD KAZEM, Mohammadnejad, Ahad, RISMANCHI, SANAZ, TAHERI, MOHAMMAD, & SHAMS, GHOLAMREZA. (2019). Carcinogenic and cytotoxic effects of Diazinon in the mouse: an in vivo and in vitro study. IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 3(3 (11) ), 159-168. SID. https://sid.ir/paper/394792/en

Vancouver: Copy

ASGHARI MOZHGAN, Sadeghi Hashjin Goudarz, Golabchifar Aliakbar, KOOHI MOHAMMAD KAZEM, Mohammadnejad Ahad, RISMANCHI SANAZ, TAHERI MOHAMMAD, SHAMS GHOLAMREZA. Carcinogenic and cytotoxic effects of Diazinon in the mouse: an in vivo and in vitro study. IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2019;3(3 (11) ):159-168. Available from: https://sid.ir/paper/394792/en

IEEE: Copy

MOZHGAN ASGHARI, Goudarz Sadeghi Hashjin, Aliakbar Golabchifar, MOHAMMAD KAZEM KOOHI, Ahad Mohammadnejad, SANAZ RISMANCHI, MOHAMMAD TAHERI, and GHOLAMREZA SHAMS, “Carcinogenic and cytotoxic effects of Diazinon in the mouse: an in vivo and in vitro study,” IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, vol. 3, no. 3 (11) , pp. 159–168, 2019, [Online]. Available: https://sid.ir/paper/394792/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops